Fetal-derived macrophages persist and sequentially maturate in ovaries after birth in mice
| Autor: | Emmi Lokka, Pia Rantakari, Heli Jokela, Sofia Tyystjärvi, Heidi Gerke, Marko Salmi, Miikka Kiviranta, Kati Elima |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Immunology Cell Inflammation Cell fate determination Major histocompatibility complex Monocytes 03 medical and health sciences Mice 0302 clinical medicine Fetus medicine Immunology and Allergy Animals Homeostasis Cell Lineage Yolk sac Mice Knockout biology Monocyte Macrophages Embryogenesis Ovary Membrane Proteins Cell Differentiation Embryonic stem cell Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure biology.protein Female medicine.symptom Single-Cell Analysis 030215 immunology |
| Zdroj: | European journal of immunologyReferences. 50(10) |
| ISSN: | 1521-4141 |
| Popis: | Macrophages, which are highly diverse in different tissues, play a complex and vital role in tissue development, homeostasis, and inflammation. The origin and heterogeneity of tissue-resident monocytes and macrophages in ovaries remains unknown. Here we identify three tissue-resident monocyte populations and five macrophage populations in the adult ovaries using high-dimensional single cell mass cytometry. Ontogenic analyses using cell fate mapping models and cell depletion experiments revealed the infiltration of ovaries by both yolk sac and fetal liver-derived macrophages already during the embryonic development. Moreover, we found that both embryonic and bone marrow-derived macrophages contribute to the distinct ovarian macrophage subpopulations in the adults. These assays also showed that fetal-derived MHC II-negative macrophages differentiate postnatally in the maturing ovary to MHC II-positive cells. Our analyses further unraveled that the developmentally distinct macrophage types share overlapping distribution and scavenging function in the ovaries under homeostatic conditions. In conclusion, we report here the first comprehensive analyses of ovarian monocytes and macrophages. In addition, we show that the mechanisms controlling monocyte immigration, the phenotype of different pools of interstitial macrophages, and the interconversion capacity of fetal-derived macrophages in ovaries are remarkably different from those seen in other tissue niches. |
| Databáze: | OpenAIRE |
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