Biosurfactant production by Pseudomonas strains isolated from floral nectar
| Autor: | Kris Willems, Christel Verreth, I. Van de Voorde, Z. Ben Belgacem, Bart Lievens, S. Bijttebier, Stefan Ruyters, Hans Jacquemyn, Guido Aerts, S. Voorspoels |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial food.ingredient biology Plant Nectar Sunflower oil Pseudomonas Pseudomonas jessenii Molecular Sequence Data food and beverages Pseudomonas fluorescens General Medicine Flowers biology.organism_classification 16S ribosomal RNA Applied Microbiology and Biotechnology Surface-Active Agents Vegetable oil food Pseudomonas fragi RNA Ribosomal 16S Botany Nectar Phylogeny Biotechnology |
| Zdroj: | Journal of applied microbiology. 118(6) |
| ISSN: | 1365-2672 |
| Popis: | Aims To screen and identify biosurfactant-producing Pseudomonas strains isolated from floral nectar; to characterize the produced biosurfactants; and to investigate the effect of different carbon sources on biosurfactant production. Methods and Results Four of eight nectar Pseudomonas isolates were found to produce biosurfactants. Phylogenetic analysis based on three housekeeping genes (16S rRNA gene, rpoB and gyrB) classified the isolates into two groups, including one group closely related to Pseudomonas fluorescens and another group closely related to Pseudomonas fragi and Pseudomonas jessenii. Although our nectar pseudomonads were able to grow on a variety of water-soluble and water-immiscible carbon sources, surface active agents were only produced when using vegetable oil as sole carbon source, including olive oil, sunflower oil or waste frying sunflower oil. Structural characterization based on thin layer chromatography (TLC) and ultra high performance liquid chromatography-accurate mass mass spectrometry (UHPLC-amMS) revealed that biosurfactant activity was most probably due to the production of fatty acids (C16:0; C18:0; C18:1 and C18:2), and mono- and diglycerides thereof. Conclusions Four biosurfactant-producing nectar pseudomonads were identified. The active compounds were identified as fatty acids (C16:0; C18:0; C18:1 and C18:2), and mono- and diglycerides thereof, produced by hydrolysis of triglycerides of the feedstock. Significance and Impact of the Study Studies on biosurfactant-producing micro-organisms have mainly focused on microbes isolated from soils and aquatic environments. Here, for the first time, nectar environments were screened as a novel source for biosurfactant producers. As nectars represent harsh environments with high osmotic pressure and varying pH levels, further screening of nectar habitats for biosurfactant-producing microbes may lead to the discovery of novel biosurfactants with broad tolerance towards different environmental conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text