Rapid PCR Method for the Selection of 1,3-Pentadiene Non-Producing Debaryomyces hansenii Yeast Strains
| Autor: | María-Isabel de Silóniz, Petra Wrent, Eva-María Rivas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
sorbate
Preservative Biotecnología Health (social science) Plant Science Bacterial growth Microbiología lcsh:Chemical technology Health Professions (miscellaneous) Microbiology 03 medical and health sciences chemistry.chemical_compound Debaryomyces hansenii lcsh:TP1-1185 Food science Gene 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry Food preservation spoilage-yeast biology.organism_classification Yeast strains-selection Pcr method food-preservation Sorbic acid Food Science 1 3-pentadiene |
| Zdroj: | Foods, Vol 9, Iss 2, p 162 (2020) E-Prints Complutense: Archivo Institucional de la UCM Universidad Complutense de Madrid Foods Volume 9 Issue 2 E-Prints Complutense. Archivo Institucional de la UCM instname |
| ISSN: | 2304-8158 |
| Popis: | To prevent microbial growth and its consequences, preservatives such as sorbic acid or its salts, commonly known as sorbates, are added to foods. However, some moulds and yeasts are capable of decarboxylating sorbates and producing 1,3-pentadiene. This is a volatile compound with an unpleasant &ldquo petroleum-like &ldquo odour, which causes consumer rejection of the contaminated products. In this work, we studied the production of 1,3-pentadiene in 91 strains of the yeast Debaryomyces hansenii, and we found that nearly 96% were able to produce this compound. The sequence of the FDC1Dh gene was analysed showing differences between 1,3-pentadiene producer (P) and non-producer (NP) strains. A specific PCR assay with degenerated primers based on the gene sequence was developed to discern NP and P strains. It was tested on D. hansenii strains and on some physiologically related species frequently isolated from foods, such as D. fabrii, D. subglobosus and Meyerozyma guillermondii. This method could be applied for the selection of NP D. hansenii strains, useful in biotechnological food production and as a biocontrol agent. |
| Databáze: | OpenAIRE |
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