Circulating plasma microRNAs in colorectal neoplasia: A pilot study in assessing response to therapy
| Autor: | Susan Galandiuk, Uri Netz, Jacob Hallion, Jianmin Pan, Mason Paas, Stephen J. O'Brien, Vincent Stephen, Kayla Feagins, Shesh N. Rai, James R. Burton, Campbell Bishop |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Original article Future studies Response to therapy Colorectal cancer lcsh:RC254-282 Serology Resection 03 medical and health sciences 0302 clinical medicine Mirna expression Internal medicine microRNA mental disorders medicine cardiovascular diseases Surveillance business.industry nutritional and metabolic diseases medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Colorectal surgery digestive system diseases 030104 developmental biology 030220 oncology & carcinogenesis Colorectal advanced adenoma business |
| Zdroj: | Translational Oncology Translational Oncology, Vol 14, Iss 1, Pp 100962-(2021) |
| ISSN: | 1936-5233 |
| Popis: | Highlights • This pilot study examines a microRNA panel as a biomarker for response to surgical resection in colorectal cancer or colorectal advanced adenoma. • A panel of 11 microRNAs was developed through screening and previous studies. • Six miRNA are significantly increased following colorectal cancer resection. • Three miRNA are significantly increased following colorectal advanced adenoma resection. • The results of this study suggest that serum microRNA expression could be followed as a marker for response to therapy. Introduction Current serological surveillance markers to monitor colorectal cancer (CRC) or colorectal advanced adenomas (CAA) are hampered by poor sensitivity and specificity. The aim of this study is to identify and validate a panel of plasma microRNAs which change in expression after resection of such lesions. Methods A prospectively maintained colorectal surgery database was queried for patients in whom both pre- and post-procedural serum samples had been obtained. An initial screening analysis of CRC and CAA patients (5 each) was conducted using screening cards for 380 miRNAs. Four identified miRNAs were combined with a previously described panel of 7 miRNAs that were diagnostically predictive of CRC and CAA. Differential miRNA expression was assessed using quantitative real-time polymerase chain reaction(qRT-PCR). Results Fifty patients were included (n = 27 CRC, n = 23 CAA). There was no difference in age, gender, or race profile of CRC patients compared to CAA patients. Six miRNA were significantly increased after CRC resection (miR-324, let7b, miR-454, miR-374a, miR-122, miR-19b, all p |
| Databáze: | OpenAIRE |
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