Preclinical Investigation of Methylene Blue-mediated Antimicrobial Photodynamic Therapy on Leishmania Parasites Using Real-Time Bioluminescence
| Autor: | Ismael P. Sauter, Martha S. Ribeiro, Fernanda V. Cabral, Mauro Cortez, Caetano P. Sabino, Jesica Ayelen Dimmer |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
030103 biophysics CUTANEOUS LEISHMANIASIS Luminescence medicine.medical_treatment 030106 microbiology Antiprotozoal Agents Leishmaniasis Cutaneous Photodynamic therapy Biochemistry Microbiology Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound Mice Biología Celular Microbiología Cutaneous leishmaniasis MEDICAMENTO medicine Bioluminescence Animals Red light Physical and Theoretical Chemistry AZUL DE METILENO Leishmania Leishmania amazonensis Mice Inbred BALB C Photosensitizing Agents biology Dose-Response Relationship Drug LED General Medicine Antimicrobial medicine.disease biology.organism_classification Methylene Blue chemistry Photochemotherapy Female RED LIGHT CIENCIAS NATURALES Y EXACTAS Methylene blue |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1751-1097 |
| Popis: | Cutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as a promising treatment for CL. In this work, we evaluated methylene blue (MB)-mediated APDT (MB-APDT) on Leishmania amazonensis in vitro and in vivo by bioluminescence technique. In vitro, MB-APDT was performed using a red LED (λ = 660 ± 11 nm, 100 mW cm−2) and MB (100 µm) at different light doses. In vivo, mice were infected and 4 weeks later, randomly divided into three groups: control, APDT 1 (single session) and APDT 2 (two sessions of MB-APDT). MB was used at 100 µm and energy dose was established at 150 J cm−2. Parasite burden, lesion size and pain were evaluated weekly for 4 weeks. In vitro, lethal dose for 90% parasite inactivation was achieved at 48.8 J cm−2. In vivo, although APDT 1 and APDT 2 groups have showed similar parasite burden after 4 weeks, two sessions were clinically better, especially considering the inflammatory process associated to CL. Our findings reinforce MB-APDT as a cost-effective treatment to combat CL. Fil: Cabral, Fernanda V.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil Fil: Sabino, Caetano P.. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil Fil: Dimmer, Jesica Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Sauter, Ismael P.. Universidade do Sao Paulo. Instituto de Ciencias Biomedicas; Brasil Fil: Cortez, Mauro J.. Universidade do Sao Paulo. Instituto de Ciencias Biomedicas; Brasil Fil: Simoes Ribeiro, Martha. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energéticas e Nucleares; Brasil |
| Databáze: | OpenAIRE |
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