Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail
| Autor: | David Huss, Greg Poynter, Rusty Lansford |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
animal structures
biology Transgene Lentivirus Cell Culture Techniques Gene Transfer Techniques Coturnix biology.organism_classification General Biochemistry Genetics and Molecular Biology Quail Genetically modified organism Viral vector Cell biology Animals Genetically Modified Germ Cells Cell culture biology.animal embryonic structures Animals Blastoderm Ovum |
| Zdroj: | Cold Spring Harbor Protocols. 2009:pdb.prot5118 |
| ISSN: | 1559-6095 |
| DOI: | 10.1101/pdb.prot5118 |
| Popis: | INTRODUCTIONThis protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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