Validation of Alternative Transcript Splicing in Chicken Lines that Differ in Genetic Resistance to Marek’s Disease
| Autor: | Muhammet Kaya, Likit Preeyanon, Hans H. Cheng, Jerry B. Dodgson |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Candidate gene Marek's disease biology Alternative splicing Bioengineering biology.organism_classification Molecular biology DNA sequencing Alternative Splicing 03 medical and health sciences Exon 030104 developmental biology 0302 clinical medicine RNA splicing Marek Disease Animals Animal Science and Zoology Chickens Gene 030217 neurology & neurosurgery INDEL Mutation Disease Resistance Biotechnology |
| Zdroj: | Animal Biotechnology. 27:238-244 |
| ISSN: | 1532-2378 1049-5398 |
| Popis: | Utilizing RNA-seq data, 1,574 candidate genes with alternative splicing were previously identified between two chicken lines that differ in Marek's disease (MD) genetic resistance under control and Marek's disease virus infection conditions. After filtering out 1,530 genes with splice variants in the first or last exon, 44 genes were screened for possible exon loss or gain using PCR and gel electrophoresis. Consequently, 7 genes exhibited visually detectable differential expression of splice variants between lines 6 (MD resistant) and 7 (MD susceptible), and the resultant PCR products verified by DNA sequencing. Birds from inbred line 6 have transcripts that preferentially retain an exon compared to line 7 chickens for ITGB2, SGPL1, and COMMD5. Birds from inbred line 7 have alleles that preferentially retain an exon compared to line 6 for MOCS2. CCBL2 exon 1a is absent and ATAD1 exon 2 is truncated by 87 nucleotides in transcripts expressed by line 7 compared to those from line 6. For CHTF18, line 6 transcripts have an indel mutation with 7 additional nucleotides in exon 21 compared to line 7. The current study validates 7 genes with alternatively spliced isomers between the two chicken lines, which helps provide potential underlying mechanisms for the phenotypic differences. |
| Databáze: | OpenAIRE |
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