Genome‐scale, single‐cell‐type resolution of microRNA activities within a whole plant organ
| Autor: | Olivier Voinnet, Matthias Hirsch-Hoffmann, Nicolás G. Bologna, Christopher A. Brosnan, Alexis Sarazin, PeiQi Lim |
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| Přispěvatelé: | European Research Council, EMBO, Swiss National Science Foundation, Bologna, Nicolas Gerardo, Voinnet, Olivier, Bologna, Nicolas Gerardo [0000-0002-2161-7910], Voinnet, Olivier [0000-0001-6982-9544] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
AGO1
Cell type Targetome Meristem Arabidopsis Computational biology Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant microRNA Gene expression miRoot translatome targetome medicine Molecular Biology Organism 030304 developmental biology Regulation of gene expression 0303 health sciences General Immunology and Microbiology Arabidopsis Proteins General Neuroscience Articles Argonaute Complex cell biology.organism_classification MicroRNAs medicine.anatomical_structure RNA Plant Argonaute Proteins Single-Cell Analysis 030217 neurology & neurosurgery |
| Zdroj: | The EMBO Journal, 38 (12) EMBO J Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Loaded into ARGONAUTE(AGO) proteins, eukaryotic micro(mi)RNAs regulate gene expression via cleavage, translational repression, and/or accelerated decay of sequence‐complementary target transcripts. Despite their importance in development, cell identity maintenance and stress responses, how individual miRNAs contribute to spatial gene regulation within the complex cell mosaics formed in tissues/organs has remained inaccessible in any organism to date. We have developed a non‐invasive methodology to examine, at single‐cell‐type resolution, the AGO‐loading and activity patterns of entire miRNA cohorts in intact organs, applied here to the Arabidopsis root tip. A dual miRNAome–targetome analytical interface allowing intuitive data integration/visualization was developed as the basis for in‐depth investigations via single‐cell‐type experimentation. These uncovered an array of so far speculative or hitherto unknown types of spatial miRNA‐mediated gene regulation schemes, including via widespread cell‐to‐cell movement between contiguous layers of distinct identities. This study provides the proof of principle that minimally invasive, genome‐scale analysis of miRNA activities within and between single‐cell types of whole organs is achievable. CAB was supported by an EMBO long‐term fellowship (ALTF 728‐2009). Work was supported by a European Research Council Advanced Grant (Frontiers in RNAi‐II No. 323071) and a grant from the Swiss National Foundation (No. 310030B_152832) both attributed to O.V. |
| Databáze: | OpenAIRE |
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