The processing and collaborative assay of a reference endotoxin

Autor:	H.D. Hochstein, Suresh C. Rastogi, A.S. Outschoorn, D.F. Mills
Rok vydání:	1983
Předmět:	Chromatography Chemical Phenomena Fever Chemistry Analytical chemistry Limulus test Reference Standards General Biochemistry Genetics and Molecular Biology Endotoxins Multicenter study Homogeneous Escherichia coli Animals Bacterial endotoxin Statistical analysis Reference standards Limulus Test
Zdroj:	Journal of Biological Standardization. 11:251-260
ISSN:	0092-1157
DOI:	10.1016/s0092-1157(83)80013-4
Popis:	A preparation of Escherichia coli bacterial endotoxin, the latest of successive lots drawn from bulk material which has been studied in laboratory tests and in animals and humans for suitability as a reference endotoxin, has been filled and lyophilized in a large number of vials. Details of its characterization, including stability studies, are given. A collaborative assay was conducted by 14 laboratories using gelation end-points with Limulus amebocyte lysates. Approximate continuity of the unit of potency with the existing national unit was achieved. The lot was made from the single final bulk but had to be freeze-dried in five sublimators. An assessment was therefore made for possible heterogeneity. The results indicate that the lot can be used as a large homogeneous quantity. The advantages of using it widely as a standard for endotoxins are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59e275b7151febf124979fa6f33ebcea https://doi.org/10.1016/s0092-1157(83)80013-4 Zobrazit plný text záznamu