Inmunodiagnóstico de la triquinosis humana
| Autor: | Saavedra T, Hugo Schenone, Salinas P, María del Carmen Contreras, Lea Sandoval |
|---|---|
| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2001 |
| Předmět: |
ELISA IgA
Indirect hemagglutination biology business.industry diagnosis Group ii Trichinella spiralis Trichinosis medicine.disease biology.organism_classification Serum samples diagnóstico Specific antibody trichinosis Antigen triquinosis Immunology Elisa test medicine Parasitology ELISA IgG business ELISA IgM |
| Zdroj: | Boletín chileno de parasitología, Volume: 56, Issue: 3-4, Pages: 47-53, Published: JUL 2001 Boletín chileno de parasitología v.56 n.3-4 2001 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | An indirect hemagglutination test (IHAT) and an ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgG, IgM and IgA specific antibodies in 113 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences (Group I). The same tests were also performed on 110 serum samples corresponding to patients without strong evidences of having trichinosis (Group II). In Group I the corresponding sensitivities for RHAI, ELISA IgG, ELISA IgM, ELISA IgA were: 82.3-85.8-88.5 and 88.5% respectively. Seventeen patients were tested again a week after the first analysis (10 of them corresponded to negative ones), increasing the positivity: 23.5-100.0;35.3-100.0;41.2-100.0 and 41.2-100.0% for RHAI, ELISA IgG, ELISA IgM and ELISA IgA, respectively. Other two patients were followed-up for 5 years. IHAT and ELISA IgG remained positive, whereas ELISA IgM and ELISA IgA were constantly negative betweeen 17 and 32 months in one case, and between 48 and 60 months in the other (this last one had presented a severe clinic disease). In the group II, four patients were positive with IHAT, however only one for ELISA IgA, the latter also presented ELISA IgM near the cut off. The use of ELISA IgG, ELSIA IgM and ELISA IgA in the immunodiagnosis of trichinosis is discussed. |
| Databáze: | OpenAIRE |
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