Comprehensive Analysis of the Expression of Sodium/Potassium-ATPase α Subunits and Prognosis of Ovarian Serous Cystadenocarcinoma
| Autor: | Ye Xu, Ge Lou, Lan Huang, Wei Huang, Shaoyou Yang, Bing Li, Yongjian Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Cystadenocarcinoma Human Protein Atlas lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine ATP1A2 ATP1A3 Gene expression ATP1A4 Genetics medicine lcsh:QH573-671 Na+/K+-ATPase 030304 developmental biology 0303 health sciences Messenger RNA lcsh:Cytology business.industry Ovary lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Oncology Gynecology 030220 oncology & carcinogenesis Cancer research Primary Research business Adenosine triphosphate |
| Zdroj: | Cancer Cell International Cancer Cell International, Vol 20, Iss 1, Pp 1-13 (2020) |
| DOI: | 10.21203/rs.3.rs-23702/v2 |
| Popis: | Background Ovarian serous cystadenocarcinoma (OSC) is the most common and lethal gynecological cancer in women worldwide; however, biomarkers to diagnose and predict prognosis of OSC remain limited. Therefore, the present study aimed to investigate whether sodium/potassium adenosine triphosphate (Na+/K+-ATP)ase α-subunits (ATP1As) are helpful diagnostic and prognostic markers of OSC. Methods Gene expression data (RNA-Seq) of 376 patients with OSC were downloaded from The Cancer Genome Atlas (TCGA) program database. Additional databases used in our analysis included the Gene Expression Omnibus, International Cancer Genome Consortium, Genotype-Tissue Expression, the Human Protein Atlas, cBioPortal for Cancer Genomics, and Cancer Cell Line Encyclopedia. Results The expression levels of ATP1A1 and ATP1A3 were higher in OSC tissues than in normal ovarian tissues, whereas the expression levels of ATP1A2 and ATP1A4 were lower in OSC tissues than in normal ovarian tissues. Overexpression of ATP1A2 was significantly associated with a higher Federation of Gynecology and Obstetrics (FIGO) stage and histological grade. Increased mRNA expression of ATP1A3 was significantly associated with shorter overall survival (OS) and disease-specific survival (DSS) in patients with OSC, whereas higher expression of ATP1A4 was associated with favorable OS and DSS. Multivariate analysis showed that primary therapy outcome, residual tumor, and mRNA expressions of ATP1A3 and ATP1A4 were independent prognostic factors for both OS and DSS in patients with OSC. Moreover, ATP1A1 staining was abundant in tumor tissues. A high expression of ATP1A3 was significantly correlated with poor OS and DSS in the subgroup of patients aged ≥ 60 years and with FIGO stage III, histological grade G3, and TP53 mutation. Mutation frequencies of the ATP1As were 3–5%. Conclusions These results indicate that the ATP1A gene family could be potential diagnostic or prognostic markers of OSC. In addition, ATP1As may be effective therapeutic targets in the treatment of OSC. |
| Databáze: | OpenAIRE |
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