Model of bleaching and acquisition for superresolution microscopy controlled by a single wavelength
| Autor: | Alex Small |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Point spread function
Microscopy Fluorophore Materials science genetic structures business.industry Super-resolution microscopy Photobleaching Fluorescence Atomic and Molecular Physics and Optics ocis:(100.6640) Superresolution chemistry.chemical_compound Optics Förster resonance energy transfer chemistry ocis:(180.2520) Fluorescence microscopy Fluorescence microscope sense organs business Biological system Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | We consider acquisition schemes that maximize the fraction of images that contain only a single activated molecule (as opposed to multiple activated molecules) in superresolution localization microscopy of fluorescent probes. During a superresolution localization microscopy experiment, irreversible photobleaching destroys fluorescent molecules, limiting the ability to monitor the dynamics of long-lived processes. Here we consider experiments controlled by a single wavelength, so that the bleaching and activation rates are coupled variables. We use variational techniques and kinetic models to demonstrate that this coupling of bleaching and activation leads to very different optimal control schemes, depending on the detailed kinetics of fluorophore activation and bleaching. Likewise, we show that the robustness of the acquisition scheme is strongly dependent on the detailed kinetics of activation and bleaching. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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