Septin Filament Compaction Into Rings Requires the Anillin Mid2 and Contractile Ring Constriction

Autor: Federica Arbizzani, Manos Mavrakis, Marta Hoya, Juan Carlos Ribas, Sophie Brasselet, Anne Paoletti, Sergio A. Rincon
Přispěvatelé: Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), MOSAIC (MOSAIC), Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Universidad de Salamanca, Instituto de Biología Funcional y Genómica [Salamanca, Spain] (IBFG), Universidad de Salamanca-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), This work was supported by a grant from Fondation ARC to A.P. Federica Arbizzani received a fourth year PhD fellowship fromFondation ARC. S.A.R. is a Ramon y Cajal fellow from the University of Salamanca (RYC-2016-20652). This work is supported bythe grants PGC2018-094090-B-I00 and PGC2018-098924-B-I00 (Ministerio de Ciencia e12 Innovación, MICINN, Spain, and ERDF, EU), and CSI150P20 and 'Escalera de Excelencia'13 CLU-2017-03 (Junta de Castilla y León 14-20, Spain and ERDF, EU) ., ANR-17-CE13-0014,SEPTIMORF,Rôle des septines dans la morphogenèse des cellules animales(2017), ANR-11-LABX-0038,CelTisPhyBio,Des cellules aux tissus: au croisement de la Physique et de la Biologie(2011), Fondation ARC pour la Recherche sur le Cancer, Ministerio de Ciencia e Innovación (España), Junta de Castilla y León, Agence Nationale de la Recherche (France)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Cell Reports
Cell Reports, 2022, ⟨10.1016/j.celrep.2022.110722⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
Cell Reports, 2022, 39 (3), pp.110722. ⟨10.1016/j.celrep.2022.110722⟩
ISSN: 2211-1247
Popis: Septin filaments assemble into high-order molecular structures that associate with membranes, acting as diffusion barriers and scaffold proteins crucial for many cellular processes. How septin filaments organize in such structures is still not understood. Here, we used fission yeast to explore septin filament organization during cell division and its cell cycle regulation. Live-imaging and polarization microscopy analysis uncovered that septin filaments are initially recruited as a diffuse meshwork surrounding the acto-myosin contractile ring (CR) in anaphase, which undergoes compaction into two rings when CR constriction is initiated. We found that the anillin-like protein Mid2 is necessary to promote this compaction step, possibly acting as a bundler for septin filaments. Moreover, Mid2-driven septin compaction requires inputs from the septation initiation network as well as CR constriction and the β(1,3)-glucan synthase Bgs1. This work highlights that anillin-mediated septin ring assembly is under strict cell cycle control.
This work was supported by a grant from Fondation ARC to A.P. (PJA 20171206550). F.A. received a fourth-year PhD fellowship from Fondation ARC (DOC20180507063). A.P. is a member of the Labex CelTisPhyBio (ANR11-LABX-0038). S.A.R. is a Ramon y Cajal fellow from the University of Salamanca (RYC-2016-20652). This work was also supported by the grants PGC2018-094090-B-I00 and PGC2018-098924-B-I00 (Ministerio de Ciencia e Innovación, MICINN, Spain, and ERDF, EU), and CSI150P20 and “Escalera de Excelencia” CLU-2017-03 (Junta de Castilla y León 14–20, Spain and ERDF, EU) and ANR-17-CE13-0014; SEPTIMORF (Agence Nationale de la Recherche).
Databáze: OpenAIRE
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