Screening and identification of serum proteomic biomarkers for gastric adenocarcinoma
| Autor: | Yong Liang, Chunqin Pan, Chibo Liu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
medicine.medical_specialty Pathology Oncogene Apolipoprotein B biology Atrophic gastritis business.industry Cancer Articles General Medicine medicine.disease Fibrinogen Tandem mass spectrometry High-performance liquid chromatography Gastroenterology Immunology and Microbiology (miscellaneous) Internal medicine medicine biology.protein Time-of-flight mass spectrometry business medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine. 3:1005-1009 |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2012.515 |
| Popis: | The aim of the present study was to screen for possible serum biomarkers for gastric adenocarcinoma. Surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) was used to screen serum samples from 109 cases of gastric adenocarcinoma and 106 control subjects (60 healthy subjects, 30 patients with chronic superficial gastritis and 16 cases of chronic atrophic gastritis). The differentially expressed protein peaks were selected and isolated using high performance liquid chromatography (HPLC) and processed with enzyme prior to liquid chromatography-mass spectrometry tandem mass spectrometry (LC-MS/MS) analysis and data mining with software XCalibur program components BioWorks 3.2. Among the gastric cancer cases, three differentially expressed protein peaks were selected as potential serum biomarkers: the m/z peaks at 5,906.5 showed increased expression (8.53±4.33 in the cancer group, and 0.88±0.31 in the control group); the m/z peaks at 6,635.7 and 8,716.3 showed decreased expression (6.54±2.44 and 0.93±0.29, respectively, in the cancer group and 17.56±4.43 and 2.16±0.98, respectively, in the control group) (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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