Diuretic effect of extracts, fractions and two compounds 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid and 5-hydroxy-3,6,7,8,4′-pentamethoxyflavone from Rubus rosaefolius Sm. (Rosaceae) leaves in rats
| Autor: | Jairo Kenupp Bastos, Lincon Bordignon Somensi, Valdir Cechinel-Filho, Camile Cecconi Cechinel-Zanchett, Marcel Petreanu, Luisa Mota da Silva, Thaise Boeing, Rivaldo Niero, Priscila de Souza, Sérgio Faloni de Andrade |
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| Rok vydání: | 2017 |
| Předmět: |
Atropine
Male medicine.medical_specialty FOLHAS (PLANTAS) medicine.medical_treatment Indomethacin Diuresis Prostaglandin Muscarinic Antagonists Urine Pharmacology 030226 pharmacology & pharmacy Dinoprostone Excretion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hydrochlorothiazide Internal medicine medicine Animals Cyclooxygenase Inhibitors Rats Wistar Diuretics Plant Extracts General Medicine Flavones Triterpenes Plant Leaves NG-Nitroarginine Methyl Ester Endocrinology chemistry 030220 oncology & carcinogenesis Urea Nitric Oxide Synthase Diuretic Rubus medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Although diuretics have been widely used to treat hypertension along with others cardiovascular and renal disorders, no scientific data have been recorded to support the diuretic properties of Rubus rosaefolius Sm. (Rosaceae), a plant popularly used in Brazil to treat hypertension. Male Wistar rats were orally treated with: vehicle; hydrochlorothiazide; aqueous (AERR) and methanolic (MERR) extracts; dichloromethane (DCM), hexane (HEX) and ethyl acetate (EA) fractions; and the isolated compounds 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid (TUA) and 5-hydroxy-3,6,7,8,4'-pentamethoxyflavone (PMF). At the end of the experiment (after 8 or 24 h), urine volume and other urine or plasma parameters were measured. AERR and MERR, at 100 and 30 mg/kg, respectively, induced diuretic, natriuretic and kaliuretic effect. Additionally, the DCM and HEX, but not EA, at 10 mg/kg, also increased urine volume and Na+ and K+ excretion. Both active constituents, TUA and PMF, at doses of 1 and 3 mg/kg, showed an augmented diuretic and natriuretic index. While TUA revealed a kaliuretic action, PMF did not interfere with potassium excretion. The compounds increased urinary creatinine, but not urea, levels. TUA was able to decrease calcium excretion, as well as HCTZ, while PMF effect was associated with increased urinary prostaglandin E2 levels. The non-selective muscarinic receptor antagonist (atropine) prevented TUA-induced diuresis. In addition, indomethacin (a cyclooxygenase inhibitor) and atropine, exhibited the ability to block the diuretic effects prompted by PMF. Our study demonstrates the diuretic effect of extracts, fractions and two natural compounds obtained from R. rosaefolius leaves in rats. |
| Databáze: | OpenAIRE |
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