Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system inEscherichia coli
| Autor: | Robert K. Poole, Rohani Hashim, Guanghui Wu, Steven J. Beard, Marie R.B. Binet, Martin N. Hughes |
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| Rok vydání: | 2000 |
| Předmět: |
Transposable element
Mutant chemistry.chemical_element Zinc medicine.disease_cause Microbiology Phosphates chemistry.chemical_compound Escherichia coli Genetics medicine Molecular Biology Ion Transport biology Chromosome Mapping Drug Resistance Microbial Membrane transport Phosphate biology.organism_classification Enterobacteriaceae Mutagenesis Insertional chemistry Biochemistry DNA Transposable Elements bacteria Carrier Proteins Bacteria |
| Zdroj: | FEMS Microbiology Letters. 184:231-235 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2000.tb09019.x |
| Popis: | A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM ZnSO(4) during growth in Luria-Bertani medium. |
| Databáze: | OpenAIRE |
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