Gene Expression Patterns of Normal Human Skin, Actinic Keratosis, and Squamous Cell Carcinoma
| Autor: | Sheldon Sebastian, R. Steven Padilla, Richard A. Larson, Zeyu Jiang, Ingo Nindl |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Skin Neoplasms Keratosis Biopsy Human skin Dermatology medicine Humans Basal cell carcinoma Oligonucleotide Array Sequence Analysis Skin integumentary system medicine.diagnostic_test business.industry Actinic keratosis Cancer General Medicine medicine.disease Keratosis Actinic Gene expression profiling stomatognathic diseases Gene Expression Regulation Epidermoid carcinoma Skin biopsy Carcinoma Squamous Cell Disease Progression RNA Female business |
| Zdroj: | Archives of Dermatology. 146 |
| ISSN: | 0003-987X |
| DOI: | 10.1001/archdermatol.2009.378 |
| Popis: | Objectives To identify and compare the gene expression profiles of actinic keratosis (AK) and squamous cell carcinoma (SCC) and to further clarify critical genetic alterations in the evolution of SCC from normal sun-damaged human skin. Design Observational study. Setting University practice. Patients Skin biopsy specimens were obtained from 16 patients. The specimens included 14 normal non–sun-exposed skin samples, 14 normal sun-exposed skin samples, 5 AKs, and 15 cutaneous SCCs. Main Outcome Measures Gene expression profiles from normal non–sun-exposed skin, normal sun-exposed skin, AKs, and SCCs. Results Using a highly astringent shrunken centroid threshold of 6.52 and the prediction analysis of microarrays, we identified 89 unique genes that most likely contribute to the molecular evolution of SCC. Our model was cross-validated using data from a separate study and clearly distinguishes between skin tumors (AK and SCC) and normal skin independent of sun exposure. Genes that were upregulated in AK and SCC were downregulated in normal skin, and genes that were downregulated in AK and SCC were upregulated in normal skin. Conclusions The finding of similar differentially expressed genes in AK and SCC confirms that AK is a precursor lesion of SCC and indicates that they are closely related genetically. Clear elucidation of these relationships will be critical to improving therapeutic approaches. |
| Databáze: | OpenAIRE |
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