Absence of occult hepatitis B virus infection in sera of diabetic children and adolescents following hepatitis B vaccination
| Autor: | Hafez Abdelhafeez, Gamal El-Didamony, Mohamed Morsy, Amr Elmestikawy, Abdallah Mahmoud Abdallah, Alaa Hashim, Nilly M George, Ashraf Elbahrawy, Ahmed Elwassief, Heba Elrashidy, Ahmed Alashker, Mohamed Mostafa, Abdel-Gawad Saeid Mohammad |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty endocrine system diseases Adolescent Immunology Enzyme-Linked Immunosorbent Assay medicine.disease_cause Gastroenterology Polymerase Chain Reaction Virus Diabetes Complications Antigen Internal medicine Diabetes mellitus medicine Prevalence Immunology and Allergy Humans Hepatitis B Vaccines Hepatitis B Antibodies Child Pharmacology Hepatitis B virus biology business.industry Hepatitis B medicine.disease Vaccination Titer DNA Viral biology.protein Egypt Female Antibody business Research Paper |
| Zdroj: | Human vaccinesimmunotherapeutics. 10(8) |
| ISSN: | 2164-554X |
| Popis: | The prevalence of occult hepatitis B (HB) infection (OBI) in HB-vaccinated diabetic children has not yet been tested. Here, we aimed to determine the prevalence of OBI among HB-vaccinated children and adolescents with insulin-dependent diabetes mellitus (IDDM).Eighty-seven (51.2%) children had a titer for antibodies to HB surface antigen (anti-HBs) of10 IU/L. These included 44 (70%) IDDM children and 43 (40.2%) healthy children. Eighty-three (48.8%) children had an anti-HBs titer of ≥10 IU/L; they included 19 (30%) with IDDM and 64 (59.8%) healthy children. None of the enrolled children (n = 170) were reactive for total antibody to HB core antigen (anti-HBc) as determined by enzyme-linked immunosorbent assay. HB virus DNA was not detected in HB-vaccinated IDDM or healthy children and adolescents.An amount of 170 HBsAg-negative sera samples from HB-vaccinated children and adolescents was included. They were classified into the IDDM group (n = 63) and the healthy control group (n = 107). HBsAg, anti-HBc, and anti-HBs were tested by enzyme-linked immunosorbent assay, and HB virus DNA was tested by nested polymerase chain reaction using 3 pairs of surface, core, and X genes.Primary HB vaccination confers long-term protection against OBI in Egyptian diabetic children and adolescents. However, the number of cases tested in this study was relatively low, and further studies and long-term follow-up of large populations are needed to draw solid and convincing conclusions. |
| Databáze: | OpenAIRE |
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