Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases
| Autor: | Daniel N. Frank, Allison L. St. Amand, Edgar C. Boedeker, Noam Harpaz, Robert A. Feldman, Norman R. Pace |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Crohn's disease
Multidisciplinary biology Firmicutes Molecular Sequence Data Faecalibacterium prausnitzii Disease Biological Sciences Inflammatory Bowel Diseases medicine.disease biology.organism_classification Ulcerative colitis Inflammatory bowel disease digestive system diseases Small intestine Pathogenesis medicine.anatomical_structure RNA Ribosomal Immunology medicine Humans Phylogeny |
| Zdroj: | Proceedings of the National Academy of Sciences. 104:13780-13785 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0706625104 |
| Popis: | The two primary human inflammatory bowel diseases, Crohn's disease (CD) and ulcerative colitis (UC), are idiopathic relapsing disorders characterized by chronic inflammation of the intestinal tract. Although several lines of reasoning suggest that gastrointestinal (GI) microbes influence inflammatory bowel disease (IBD) pathogenesis, the types of microbes involved have not been adequately described. Here we report the results of a culture-independent rRNA sequence analysis of GI tissue samples obtained from CD and UC patients, as well as non-IBD controls. Specimens were obtained through surgery from a variety of intestinal sites and included both pathologically normal and abnormal states. Our results provide comprehensive molecular-based analysis of the microbiota of the human small intestine. Comparison of clone libraries reveals statistically significant differences between the microbiotas of CD and UC patients and those of non-IBD controls. Significantly, our results indicate that a subset of CD and UC samples contained abnormal GI microbiotas, characterized by depletion of commensal bacteria, notably members of the phyla Firmicutes and Bacteroidetes . Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|