Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity
| Autor: | Zhixiao Luo, Dengke Jia, Yuanyang Li, Leiqi Zhu, Mengzhen Xue, Yan Lei, Silong Chen, Guangfu Xu, Fangqi Xia, Yaqi Wang |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Aging White adipose tissue Mitochondrion Astragaloside IV β‐oxidation chemistry.chemical_compound Mice fat catabolism Internal medicine energy metabolism medicine Lipolysis Animals Triglyceride Chemistry respiratory quotient Fatty Acids Lipid metabolism Cell Biology Original Articles Saponins Lipid Metabolism Triterpenes Mitochondria Mice Inbred C57BL Mitochondrial respiratory chain Endocrinology Liver Ageing ageing Ketone bodies Molecular Medicine Original Article |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 |
| Popis: | Astragaloside IV (AST) is a major bioactive compound of Radix Astragali with medical and health benefits. Previous studies have found that AST can reduce the body weights of high‐fat diet fed mice. However, the effect of AST on fat metabolism of ageing mice is unclear. In this study, naturally ageing mice were administered intragastrically with AST at 30 mg/kg/day (ageing + AST‐L group) and 90 mg/kg/day (ageing + AST‐H group) for 16–20 months. Adult (4 months old) and ageing mice were given 1% sodium carboxyl methylcellulose as vehicle. Energy metabolism‐related biological parameters of living mice were examined. Moreover, mRNA and protein levels of key enzymes/proteins involved in triglyceride (TG) lipolysis, fatty acid β‐oxidation (FAO), ketone body (KB) production and mitochondrial respiratory chain were also examined after sacrifice. Results demonstrated that treatment with AST significantly reduced body weight, white fat and liver/body weight ratio of ageing mice, significantly reduced serum/hepatic TG levels, respiratory quotient, promoted fatty acid mobilization in white adipose tissue, mitochondrial FAO and KB production and mitochondrial biosynthesis/functions in the liver of ageing mice. AST also up‐regulated the expression of phosphorylated AMP‐activated protein kinase, acetyl‐CoA carboxylase, acetyl‐coenzyme A synthetase, carnitine palmitoyltransferase 1a/1b, enoyl coenzyme A hydratase‐short chain, acyl‐CoA dehydrogenase medium chain and mitochondrial 3‐hydroxy‐3‐methylglutaryl‐CoA synthase‐2 involved in fat metabolism. These results indicated that mitochondrial activity could be the target of AST to treat abnormal fat metabolism during ageing. |
| Databáze: | OpenAIRE |
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