Hyaluronan polymeric micelles for topical drug delivery
| Autor: | Kristina Nešporová, Daniela Šmejkalová, Veronika Machalová, Gloria Huerta-Angeles, Martin Čepa, Dominika Luptáková, Eva Achbergerová, Martina Hermannová, Tomáš Muthný, Vladimír Velebný, Marek Svoboda |
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| Rok vydání: | 2016 |
| Předmět: |
Drug
Adult Polymers and Plastics Polymers Swine media_common.quotation_subject Skin Absorption Skin Cream Nanotechnology 02 engineering and technology In Vitro Techniques 010402 general chemistry 01 natural sciences Micelle Cell Line chemistry.chemical_compound Dermis In vivo Hyaluronic acid Materials Chemistry Stratum corneum medicine Animals Humans Transcellular Hyaluronic Acid Micelles media_common Drug Carriers Organic Chemistry Penetration (firestop) Middle Aged 021001 nanoscience & nanotechnology 3. Good health 0104 chemical sciences Drug Liberation medicine.anatomical_structure chemistry Biophysics 0210 nano-technology |
| Zdroj: | Carbohydrate polymers. 156 |
| ISSN: | 1879-1344 |
| Popis: | Nanosized materials offer promising strategy for topical drug delivery due to their enhancing effect on drug percutaneous transport across the stratum corneum barrier. In this work, polymeric micelles made from hydrophobized hyaluronic acid (HA) were probed for skin delivery. Compared to non-polymeric micelle solutions containing similar drug amount, in vitro skin penetration analysis indicated 3 times larger deposition of drug in the epidermis and 6 times larger drug deposition in the dermis after 5h of topical treatment in Franz diffusion cells. The drug deposition was further increased with prolonged time of topical treatment. Laser confocal microscopy revealed the accumulation of both, the HA forming the vehicle and the payload, in the epidermis and dermis. Although fluorescent labeling of the HA would suggest co-transport of the HA and the drug, loading FRET pair dyes in the micellar core clearly demonstrated gradual micelle disruption with increasing skin depth. Transcellular penetration was the predominant pathway for the loaded drug. The HA polymeric micelles also demonstrated increased bioactivity of loaded compound in vitro and in vivo. In addition, the loaded micelles were found to be stable in cream formulations and thus they have great potential for topical applications for cosmetic and pharmaceutical purposes. |
| Databáze: | OpenAIRE |
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