Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives
| Autor: | Ines Willershausen, Andreas Taubmann, Sarah Al-Maawi, Christian Walter, Lina Gölz, Bernd Kaina |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Dental Stress Analysis Biocompatibility Orthodontic Brackets Dental Cements Pharmacology Methacrylate medicine.disease_cause DPIHP 03 medical and health sciences 0302 clinical medicine In vivo Adhesives Materials Testing medicine Cytotoxic T cell Humans ddc:610 Cytotoxicity General Dentistry Chemistry Dental Bonding 030206 dentistry In vitro Resin Cements γH2AX assay 030104 developmental biology Orthodontic adhesives Methacrylates Original Article Adhesive Shear Strength Genotoxicity DNA Damage |
| Zdroj: | Clinical Oral Investigations |
| ISSN: | 1436-3771 1432-6981 |
| Popis: | Objectives The biocompatibility of methacrylate-based adhesives is a topic that is intensively discussed in dentistry. Since only limited evidence concerning the cyto- and genotoxicity of orthodontic adhesives is available, the aim of this study was to measure the genotoxic potential of seven orthodontic methacrylate-based adhesives. Materials and methods The XTT assay was utilized to determine the cytotoxicity of Assure Plus, Assure Bonding Resin, ExciTE F, OptiBond Solo Plus, Scotchbond Universal Adhesive, Transbond MIP, and Transbond XT after an incubation period of 24 h on human gingival fibroblasts. We also performed the γH2AX assay to explore the genotoxic potential of the adhesives within cytotoxic dose ranges after an incubation period of 6 h. Results The XTT assay showed a concentration-dependent reduction in cell viability. The decrease in cellular viability was in the same dose range most significant for Assure Plus, rendering it the adhesive material with the highest cytotoxicity. Employing the γH2AX assay, a concentration-dependent increase in H2AX phosphorylation was detected, indicating induction of DNA damage. Conclusions For most products, a linear correlation between the material concentration and γH2AX foci was observed. The most severe effect on γH2AX focus induction was found for Transbond MIP, which was the only adhesive in the test group containing the co-initiator diphenyliodonium hexafluorophosphate (DPIHP). Clinical relevance The data indicate that orthodontic adhesives, notably Transbond MIP, bear a genotoxic potential. Since the study was performed with in vitro cultivated cells, a direct translation of the findings to in vivo exposure conditions should be considered with great diligence. |
| Databáze: | OpenAIRE |
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