Reactive Oxygen Species in Planarian Regeneration
| Autor: | Francesc Cebrià, Frank Van Belleghem, Nicky Pirotte, Rik Paesen, Susanna Fraguas, Michelle Plusquin, Karen Smeets, Marcel Ameloot, Andromeda Van Roten, Tom Artois, An-Sofie Stevens |
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| Přispěvatelé: | Department Science, RS-Research Line Resilience (part of LIRS program) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
LIMB REGENERATION
Aging NEURONAL FATE Article Subject Cellular differentiation Context (language use) In situ hybridization Biology Biochemistry Onium Compounds Schmidtea mediterranea HEAD REGENERATION Animals Regeneration lcsh:QH573-671 REAL-TIME PCR Tissue homeostasis Cell Proliferation GENE-EXPRESSION lcsh:Cytology DUGESIA-JAPONICA Regeneration (biology) NADPH-OXIDASE CENTRAL-NERVOUS-SYSTEM Acetophenones Brain Cell Differentiation Cell Biology General Medicine Anatomy Planarians SCHMIDTEA-MEDITERRANEA biology.organism_classification Neuroregeneration Immunohistochemistry Cell biology Planarian Reactive Oxygen Species STEM-CELLS Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2015 (2015) Pirotte, N, Stevens, A-S, Fraguas, S, Plusquin, M, Van Roten, A, Van Belleghem, F, Paesen, R, Ameloot, M, Cebria, F, Artois, T & Smeets, K 2015, ' Reactive Oxygen Species in Planarian Regeneration : An Upstream Necessity for Correct Patterning and Brain Formation ', Oxidative Medicine and Cellular Longevity, vol. 2015, 392476 . https://doi.org/10.1155/2015/392476 Oxidative Medicine and Cellular Longevity, 2015:392476. Hindawi Publishing Corporation |
| ISSN: | 1942-0900 |
| Popis: | Recent research highlighted the impact of ROS as upstream regulators of tissue regeneration. We investigated their role and targeted processes during the regeneration of different body structures using the planarian Schmidtea mediterranea, an organism capable of regenerating its entire body, including its brain. The amputation of head and tail compartments induces a ROS burst at the wound site independently of the orientation. Inhibition of ROS production by diphenyleneiodonium (DPI) or apocynin (APO) causes regeneration defaults at both the anterior and posterior wound sites, resulting in reduced regeneration sites (blastemas) and improper tissue homeostasis. ROS signaling is necessary for early differentiation and inhibition of the ROS burst results in defects on the regeneration of the nervous system and on the patterning process. Stem cell proliferation was not affected, as indicated by histone H3-P immunostaining, fluorescence-activated cell sorting (FACS), in situ hybridization of smedwi-1, and transcript levels of proliferation-related genes. We showed for the first time that ROS modulate both anterior and posterior regeneration in a context where regeneration is not limited to certain body structures. Our results indicate that ROS are key players in neuroregeneration through interference with the differentiation and patterning processes. Bijzonder Onderzoeks- Fonds of Hasselt University (BOF08G01. Hasselt University tUL-impulsfinanciering (project toxicology). Grant BFU2012-31701 (Spain). Agency for Innovation by Science and Technology (IWT) |
| Databáze: | OpenAIRE |
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