The protein turnover of Arabidopsis BPM1 is involved in regulation of flowering time and abiotic stress response

Autor: Pascal Genschik, Andreja Škiljaica, Nenad Malenica, Kristina Majsec, Nataša Bauer, Mateja Jagić, Esther Lechner
Přispěvatelé: Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Proteasome Endopeptidase Complex
Ubiquitin-Protein Ligases
MATH-BTB
Early flowering
Water deprivation
ABA
Abiotic stress
Elevated temperature
Green Fluorescent Proteins
Arabidopsis
Flowers
Plant Science
Protein degradation
Plant Roots
01 natural sciences
03 medical and health sciences
Gene Expression Regulation
Plant
Stress
Physiological
Genetics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Arabidopsis thaliana
MYB
ComputingMilieux_MISCELLANEOUS
biology
Arabidopsis Proteins
Gene Expression Profiling
fungi
Protein turnover
food and beverages
Signal transducing adaptor protein
General Medicine
Plants
Genetically Modified
biology.organism_classification
Cell biology
Ubiquitin ligase
030104 developmental biology
Proteolysis
Seeds
biology.protein
Pollen
Agronomy and Crop Science
Abscisic Acid
Plasmids
Transcription Factors
010606 plant biology & botany
Zdroj: Plant Molecular Biology
Plant Molecular Biology, Springer Verlag (Germany), 2020, 102 (4-5), pp.359-372. ⟨10.1007/s11103-019-00947-2⟩
ISSN: 1573-5028
0167-4412
DOI: 10.1007/s11103-019-00947-2
Popis: Protein degradation is essential in plant growth and development. The stability of Cullin3 substrate adaptor protein BPM1 is regulated by multiple environmental cues pointing on manifold control of targeted protein degradation. A small family of six MATH-BTB genes (BPM1-6) is described in Arabidopsis thaliana. BPM proteins are part of the Cullin E3 ubiquitin ligase complexes and are known to bind at least three families of transcription factors: ERF/AP2 class I, homeobox-leucine zipper and R2R3 MYB. By targeting these transcription factors for ubiquitination and subsequent proteasomal degradation, BPMs play an important role in plant flowering, seed development and abiotic stress response. In this study, we generated BPM1-overexpressing plants that showed an early flowering phenotype, resistance to abscisic acid and tolerance to osmotic stress. We analyzed BPM1-GFP protein stability and found that the protein has a high turnover rate and is degraded by the proteasome 26S in a Cullin-dependent manner. Finally, we found that BPM1 protein stability is environmentally conditioned. Darkness and salt stress triggered BPM1 degradation, whereas elevated temperature enhanced BPM1 stability and accumulation in planta.
Databáze: OpenAIRE
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