Increased Angiotensin II Type 1 Receptor Expression in Hypercholesterolemic Atherosclerosis in Rabbits
| Autor: | Paulette Mehta, Leping Shen, Dagmara Mohuczy, Hongbin Meng, Jason Mehta, M. I. Phillips, B. C. Yang |
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| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Vascular smooth muscle Arteriosclerosis Receptor expression Hypercholesterolemia Gene Expression Biology Polymerase Chain Reaction Receptor Angiotensin Type 2 Receptor Angiotensin Type 1 Cholesterol Dietary Norepinephrine chemistry.chemical_compound medicine.artery Internal medicine medicine Animals Tissue Distribution RNA Messenger Receptor Aorta Receptors Angiotensin Angiotensin II receptor type 1 Cholesterol Angiotensin II RNA-Directed DNA Polymerase medicine.disease Immunohistochemistry Acetylcholine Endocrinology chemistry Autoradiography Rabbits Cardiology and Cardiovascular Medicine |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 18:1433-1439 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.18.9.1433 |
| Popis: | Abstract —Angiotensin II (Ang II) promotes vascular smooth muscle growth and may be involved in the initiation and progression of atherosclerosis. To examine whether Ang II receptor expression in vascular tissues is altered in atherosclerosis, male New Zealand White rabbits were fed a high-cholesterol diet (1% cholesterol+4% coconut oil mixed with regular chow; hypercholesterolemic group, n=12) or regular chow (control group, n=8) for 10 weeks. At the end of this period, the serum cholesterol level in the rabbits fed the high-cholesterol diet was higher than that in the control group (3616±144 versus 30±1 mg/dL, P P 1 ) receptor expression (289±38 versus 38±18 fmol/mg, P P =NS). AT 1 receptors were localized primarily in the media and to some extent in the intima of the atherosclerotic aorta, as determined by immunohistochemistry with specific monoclonal and polyclonal AT 1 receptor antibodies. Increased synthesis of AT 1 receptor mRNA in atherosclerotic tissues was confirmed by reverse transcription–polymerase chain reaction. To evaluate the functional significance of increased AT 1 receptor expression, the constrictor response of aortic rings to Ang II was examined and found to be markedly enhanced in atherosclerotic aortic rings ( P P 1 receptor) expression in aortic tissues, which may result in altered vasoreactivity. |
| Databáze: | OpenAIRE |
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