An enzyme-chromogenic surface plasmon resonance biosensor probe for hydrogen peroxide determination using a modified Trinder's reagent
| Autor: | Isao Karube, Teru Kato, Kazuyoshi Yano, Takuo Akimoto, Yotaro Mogi, Kiyoshi Naemura, Hideaki Nakamura |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
education
Biomedical Engineering Biophysics Analytical chemistry Biosensing Techniques Ferric Compounds Adduct chemistry.chemical_compound Electrochemistry Glucose oxidase Surface plasmon resonance Hydrogen peroxide Horseradish Peroxidase Detection limit biology Chemistry Chromogenic Hydrogen Peroxide General Medicine Hydrogen-Ion Concentration Surface Plasmon Resonance Glucose Reagent Calibration biology.protein Biosensor Biotechnology Nuclear chemistry |
| Zdroj: | Biosensors and Bioelectronics. 24:455-460 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2008.04.022 |
| Popis: | An absorption-based surface plasmon resonance (SPR(Abs)) biosensor probe has been developed for simple and reproducible measurements of hydrogen peroxide using a modified Trinder's reagent (a chromogenic reagent). The reagent enabled the determination of the hydrogen peroxide concentration by the development of deep color dyes (lambda(max)=630 nm) through the oxidative coupling reaction with N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate (MAOS; C(13)H(20)NNaO(4)S.H(2)O) and 4-aminoantipyrine (4-AA) in the presence of hydrogen peroxide and horseradish peroxidase (HRP). In the present study, urea as an adduct of hydrogen peroxide for color development could be omitted from the measurement solution. The measurement solution containing 5mM hydrogen peroxide was deeply colored at a high absorbance value calculated as 46.7cm(-1) and was directly applied to the SPR(Abs) biosensing without dilution. The measurement was simply performed by dropping the measurement solution onto the surface of the SPR sensor probe, and the SPR(Abs) biosensor response to hydrogen peroxide was obtained as a reflectivity change in the SPR spectrum. After investigation of the pH profiles in the SPR(Abs) biosensor probe, a linear calibration curve was obtained between 1.0 and 50mM hydrogen peroxide (r=0.991, six points, average of relative standard deviation; 0.152%, n=3) with a detection limit of 0.5mM. To examine the applicability of this SPR(Abs) biosensor probe, 20mM glucose detection using glucose oxidase was also confirmed without influence of the refractive index in the measurement solution. Thus, the SPR(Abs) biosensor probe employing the modified Trinder's reagent demonstrated applicability to other analyte biosensing tools. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect