The human laryngeal microbiome: effects of cigarette smoke and reflux
Autor: | Marie E. Jetté, Kimberly A. Dill-McFarland, Alissa S. Hanshew, Susan L. Thibeault, Garret Suen |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Larynx Adult DNA Bacterial Male 030106 microbiology Physiology Disease medicine.disease_cause DNA Ribosomal Article Microbiology Cigarette Smoking 03 medical and health sciences Young Adult RNA Ribosomal 16S Smoke medicine Cluster Analysis Humans Helicobacter Microbiome Young adult Phylogeny Aged Multidisciplinary biology Bacteria Streptococcus Microbiota Reflux High-Throughput Nucleotide Sequencing Sequence Analysis DNA Middle Aged biology.organism_classification 3. Good health 030104 developmental biology medicine.anatomical_structure Gastroesophageal Reflux Pyrosequencing Female Acids |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Prolonged diffuse laryngeal inflammation from smoking and/or reflux is commonly diagnosed as chronic laryngitis and treated empirically with expensive drugs that have not proven effective. Shifts in microbiota have been associated with many inflammatory diseases, though little is known about how resident microbes may contribute to chronic laryngitis. We sought to characterize the core microbiota of disease-free human laryngeal tissue and to investigate shifts in microbial community membership associated with exposure to cigarette smoke and reflux. Using 454 pyrosequencing of the 16S rRNA gene, we compared bacterial communities of laryngeal tissue biopsies collected from 97 non-treatment-seeking volunteers based on reflux and smoking status. The core community was characterized by a highly abundant OTU within the family Comamonadaceae found in all laryngeal tissues. Smokers demonstrated less microbial diversity than nonsmokers, with differences in relative abundances of OTUs classified as Streptococcus, unclassified Comamonadaceae, Cloacibacterium, and Helicobacter. Reflux status did not affect microbial diversity nor community structure nor composition. Comparison of healthy laryngeal microbial communities to benign vocal fold disease samples revealed greater abundance of Streptococcus in benign vocal fold disease suggesting that mucosal dominance by Streptococcus may be a factor in disease etiology. |
Databáze: | OpenAIRE |
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