Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks
| Autor: | Sophie Charlotte Gleber, Thomas V. O'Halloran, Teresa K. Woodruff, Betty Y. Kong, Amanda R. Bayer, Emily L. Que, Reiner Bleher, Vinayak P. Dravid, Si Chen, Stefan Vogt, Francesca E. Duncan, Seth A. Garwin |
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| Rok vydání: | 2014 |
| Předmět: |
Microscopy
Electron Scanning Transmission Fluorescence-lifetime imaging microscopy General Chemical Engineering Analytical chemistry chemistry.chemical_element Zinc 010402 general chemistry 01 natural sciences Article Exocytosis 03 medical and health sciences Mice Scanning transmission electron microscopy Microscopy medicine Fluorescence microscope Animals 030304 developmental biology Fluorescent Dyes 0303 health sciences Microscopy Confocal Chemistry Vesicle General Chemistry Oocyte 0104 chemical sciences medicine.anatomical_structure Fertilization Biophysics Oocytes Female Cell Nucleus Division DNA Probes |
| Zdroj: | Nature chemistry |
| ISSN: | 1755-4349 |
| Popis: | Fertilization of a mammalian egg initiates a series of 'zinc sparks' that are necessary to induce the egg-to-embryo transition. Despite the importance of these zinc-efflux events little is known about their origin. To understand the molecular mechanism of the zinc spark we combined four physical approaches that resolve zinc distributions in single cells: a chemical probe for dynamic live-cell fluorescence imaging and a combination of scanning transmission electron microscopy with energy-dispersive spectroscopy, X-ray fluorescence microscopy and three-dimensional elemental tomography for high-resolution elemental mapping. We show that the zinc spark arises from a system of thousands of zinc-loaded vesicles, each of which contains, on average, 10(6) zinc atoms. These vesicles undergo dynamic movement during oocyte maturation and exocytosis at the time of fertilization. The discovery of these vesicles and the demonstration that zinc sparks originate from them provides a quantitative framework for understanding how zinc fluxes regulate cellular processes. |
| Databáze: | OpenAIRE |
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