Systematic tracking of altered modules identifies disrupted pathways in teratozoospermia
| Autor: | Tian Ep, Yao Wl, Jiang Xl, Zeng T, Huang Zq, Wang Gx |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Regulation of gene expression Gene Expression Profiling Computational Biology General Medicine Computational biology Teratozoospermia Biology Biological pathway Gene expression profiling 03 medical and health sciences 030104 developmental biology Gene Expression Regulation Genetics Humans Protein Interaction Maps Biomarker discovery Molecular Biology Gene Algorithms Protein Interaction Map Signal Transduction |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr.15027514 |
| Popis: | The objective of this study was to identify disrupted pathways in teratozoospermia by systematically tracking dysregulated modules in reweighted protein-protein interaction (PPI) networks. We inferred and reweighted the PPI networks of normal and teratozoospermia groups based on Spearman correlation coefficients. Modules in the PPI networks were explored via a clique-merging algorithm and altered modules were identified based on maximum weight bipartite matching. Furthermore, pathway-enrichment analyses of genes in altered modules were performed by Database for Annotation, Visualization, and Integrated Discovery (DAVID) to illuminate the biological pathways in teratozoospermia. A total of 20,102 genes were screened from the expression profile. We explored 2406 and 2101 modules in normal and disease PPI networks, respectively. Moreover, we obtained 875 altered modules by comparing modules in normal and teratozoospermia PPI networks. At P0.01, the genes involved in 2855 interactions with score changes1 were mainly enriched in 66 pathways and the genes in altered modules were enriched in 71 pathways. The activity genes (missed and added genes in the disrupted modules) were enriched in 41 common pathways. There were 36 mutual enriched pathways under the five different conditions. Moreover, the cell cycle pathway was disrupted in the first 10 pathways of each condition. This study provides a powerful biomarker discovery platform to better understand the progression of teratozoospermia by systematically tracking dysregulated modules. This method uncovered potential diagnostic and therapeutic targets of teratozoospermia. This information might lead to improved monitoring and treatment of teratozoospermia. |
| Databáze: | OpenAIRE |
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