Molecular cloning of restriction fragments and construction of a physical and genetic map of the Escherichia coli plasmid R538-1
| Autor: | N. Kirby Alton, Daniel Vapnek |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
DNA
Bacterial Genetic Linkage R Factors EcoRI DNA Recombinant DNA Restriction Enzymes Biology Molecular cloning medicine.disease_cause Molecular biology PBR322 Restriction fragment Restriction enzyme Endonuclease Plasmid Genes biology.protein medicine Escherichia coli Electrophoresis Polyacrylamide Gel Transformation Bacterial Molecular Biology |
| Zdroj: | Plasmid. 1(3) |
| ISSN: | 0147-619X |
| Popis: | A genetic and physical map of Escherichia coli plasmid R538-1 was constructed using restriction endonucleases and molecular cloning techniques. R538-1 DNA was cleaved into 12 fragments by endonuclease · R · Eco RI, 6 fragments by endonuclease R · Hin dIII, and 3 fragments by endonuclease R · Bam HI. The order of these fragments was determined by standard restriction fragment mapping techniques. Endo · R · Eco RI, endo · R · Hin dIII, endo · R · Bam HI, and endo · R · Pst I fragments obtained from R538-1 and ColE1-derived plasmids (pMB9, ColE1Ap r , and pBR322) were ligated in vitro and used to transform E. coli C600. Transformants were selected for antibiotic resistance markers carried by R538-1. Analysis of the R538-1 fragments contained in these hybrid plasmids permitted the construction of a genetic map of the R538-1 plasmid. The genetic map of this plasmid is very similar to that of plasmid R100. |
| Databáze: | OpenAIRE |
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