Mouse Embryonic Fibroblast Adipogenic Potential: A Comprehensive Transcriptome Analysis
| Autor: | Mohamed Abu-Farha, Mohammad H. Jamal, Hamad Ali, Khulood Al-Awadi, Mei ElGindi, Mohamed Al-Sayegh, Tina Chanyong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Histology
Physiology Cell Gene Expression Adipokine Adipose tissue Biology Diseases of the endocrine glands. Clinical endocrinology adipogenesis Transcriptome Mice Adipokines 3T3-L1 Cells CEBPA Adipocytes medicine CEBPB Animals QP1-981 adipocyte differentiation QH573-671 Sequence Analysis RNA Gene Expression Profiling High-Throughput Nucleotide Sequencing Cell Differentiation Mouse Embryonic Stem Cells Cell Biology Fibroblasts RC648-665 Embryonic stem cell adipose tissue Cell biology mouse embryonic fibroblast medicine.anatomical_structure Gene Expression Regulation Adipogenesis Cytology transcriptome Research Article Research Paper |
| Zdroj: | Adipocyte, Vol 10, Iss 1, Pp 1-20 (2021) Adipocyte article-version (VoR) Version of Record |
| ISSN: | 2162-397X 2162-3945 |
| DOI: | 10.1080/21623945.2020.1859789 |
| Popis: | Our understanding of adipose tissue has progressed from an inert tissue for energy storage to be one of the largest endocrine organs regulating metabolic homoeostasis through its ability to synthesize and release various adipokines that regulate a myriad of pathways. The field of adipose tissue biology is growing due to this association with various chronic metabolic diseases. An important process in the regulation of adipose tissue biology is adipogenesis, which is the formation of new adipocytes. Investigating adipogenesis in vitro is currently a focus for identifying factors that might be utilized in clinically. A powerful tool for such work is high-throughput sequencing which can rapidly identify changes at gene expression level. Various cell models exist for studying adipogenesis and has been used in high-throughput studies, yet little is known about transcriptome profile that underlies adipogenesis in mouse embryonic fibroblasts. This study utilizes RNA-sequencing and computational analysis with DESeq2, gene ontology, protein–protein networks, and robust rank analysis to understand adipogenesis in mouse embryonic fibroblasts in-depth. Our analyses confirmed the requirement of mitotic clonal expansion prior to adipogenesis in this cell model and highlight the role of Cebpa and Cebpb in regulating adipogenesis through interactions of large numbers of genes. |
| Databáze: | OpenAIRE |
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