Sample matrix is critical in the evaluation of a dry-slide bromocresol green method for human albumin
| Autor: | Michelle M. Payn, David Lawrence, Edmund J. Lamb, Roger Willis |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Immunoassay
Quality Control Blood Specimen Collection Bromocresol green biology Chemistry Clinical Biochemistry Serum albumin Analytical chemistry Albumin Human albumin General Medicine Bromcresol Green Dilution Diffusion Matrix (chemical analysis) chemistry.chemical_compound Bias External quality assessment biology.protein Humans Quantitative analysis (chemistry) Serum Albumin |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 39:311-313 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1258/0004563021902026 |
| Popis: | Background A dry-slide bromocresol green method for serum albumin consistently demonstrated negative bias in a proficiency testing scheme, particularly at low albumin concentrations. Methods and Results Albumin was measured using both a dry-slide method and an immunonephelometric method in patient serum samples and external quality assessment samples. Results obtained using the dry-slide method were lower than those obtained using immunonephelometry (mean bias - 1·0 g/L, P = 0·0039), but this difference was exaggerated in the quality assessment samples. In three other albumin preparations with assigned concentrations, recovery with the dry-slide method decreased upon dilution of the matrix. Conclusions Our results suggest that apparent under-recovery of albumin using this technology is probably due to the diffusive properties of the quality assessment sample matrix. |
| Databáze: | OpenAIRE |
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