Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal PlantDrynaria fortunei
| Autor: | Heng-Gang Xue, Chun-Ying Xue |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Scorpion Pharmaceutical Science Biology Polymerase Chain Reaction Analytical Chemistry law.invention Drynaria fortunei Intergenic region Polypodiaceae law biology.animal Drug Discovery Botany Base sequence Medicine Chinese Traditional Medicinal plants Polymerase chain reaction Pharmacology Plants Medicinal Base Sequence Traditional medicine Organic Chemistry Pseudodrynaria coronans Complementary and alternative medicine Molecular Medicine DNA Intergenic |
| Zdroj: | Planta Medica. 74:1416-1420 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-2008-1081305 |
| Popis: | DNA sequence analysis of the trnl-trnF spacer region and real-time scorpion polymerase chain reaction were exploited for their applications in the differentiation and quantification of the traditional Chinese medicinal plants Drynaria fortunei from five related adulterants: D. mollis, D. quercifolia, D. rigidula, D. sparsisora, and Pseudodrynaria coronans. The data demonstrated that variations in the trnl-trnF spacer regions were very low at the intraspecific level but extremely high at the interspecific level, meaning that they could be easily distinguished at the DNA level. The sequence difference allowed effective and reliable differentiation of D. fortunei from adulterants by real-time scorpion PCR. Furthermore, a quantification methodology was carried out to quantify D. fortunei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|