A BioID-derived proximity interactome for SARS-CoV-2 proteins
| Autor: | Valesca Anschau, Dexter Pratt, Danielle G. May, Laura Martin-Sancho, Kyle J. Roux, Kelsey L. Scott, Sophie Liu, Charles T Halfmann, Rachel J Chrisopulos, Ramon Diaz Pena, Alexandre Rosa Campos |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses Computational biology Biology medicine.disease_cause Interactome Article Public access Virology medicine Humans Biotinylation skin and connective tissue diseases Pandemics Coronavirus Human lung cancer SARS-CoV-2 fungi COVID-19 Fusion protein proximity labeling BioID TurboID interactome respiratory tract diseases body regions Infectious Diseases Proteome |
| Zdroj: | bioRxiv article-version (status) pre article-version (number) 1 Viruses; Volume 14; Issue 3; Pages: 611 |
| Popis: | The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here, the cellular impact of expressing SARS-CoV-2 viral proteins was studied by global proteomic analysis, and proximity biotinylation (BioID) was used to map the SARS-CoV-2 virus–host interactome in human lung cancer-derived cells. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are associated with SARS-CoV-2 proteins. We have established a website to host the proteomic data to allow for public access and continued analysis of host–viral protein associations and whole-cell proteomes of cells expressing the viral–BioID fusion proteins. Furthermore, we identified 66 high-confidence interactions by comparing this study with previous reports, providing a strong foundation for future follow-up studies. Finally, we cross-referenced candidate interactors with the CLUE drug library to identify potential therapeutics for drug-repurposing efforts. Collectively, these studies provide a valuable resource to uncover novel SARS-CoV-2 biology and inform development of antivirals. |
| Databáze: | OpenAIRE |
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