Chromosome 17 Aneusomy Detected by Fluorescence in Situ Hybridization in Vulvar Squamous Cell Carcinomas and Synchronous Vulvar Skin
| Autor: | Angela Rohwedder, Vincent L. Wilson, J. Andrew Carlson, Tien Anh Tran, Kara Healy, John H. Malfetano, Jeffrey S. Ross |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Vulvar Squamous Cell Carcinoma Gene Dosage Lichen sclerosus Biology Pathology and Forensic Medicine Vulva Image Processing Computer-Assisted medicine Humans In Situ Hybridization Fluorescence Aged Image Cytometry Skin Vulvar Diseases Aged 80 and over Vulvar neoplasm Polysomy Vulvar Neoplasms integumentary system DNA Neoplasm Middle Aged Aneuploidy Vulvar intraepithelial neoplasia medicine.disease female genital diseases and pregnancy complications stomatognathic diseases Ki-67 Antigen Lichen Sclerosus et Atrophicus medicine.anatomical_structure Epidermoid carcinoma Carcinoma Squamous Cell Female Precancerous Conditions Regular Articles Chromosomes Human Pair 17 |
| Zdroj: | The American Journal of Pathology. 157:973-983 |
| ISSN: | 0002-9440 |
| Popis: | Vulvar squamous cell carcinoma (SCC) affects a spectrum of women with granulomatous vulvar diseases, human papillomavirus (HPV) infections, and chronic inflammatory vulvar dermatoses. To determine whether there is evidence of chromosomal instability occurring in synchronous skin surrounding vulvar SCCs, we investigated abnormalities in chromosome 17 copy number. Samples of SCC, vulvar intraepithelial neoplasia (VIN), and surrounding vulvar skin were obtained from all vulvar excisions performed for squamous neoplasia at Albany Medical College from 1996 to 1997. Histological categorization, fluorescent in situ hybridization (FISH) for the alpha satellite region of chromosome 17, DNA content by image analysis, and Ki-67 labeling were evaluated. Controls of normal vulvar skin not associated with cancer were used for comparison. One hundred ten specimens were obtained from 33 patients with either SCC or VIN 3 and consisted of 49 neoplastic, 52 nonneoplastic, and 9 histologically normal vulvar skin samples. The majority of SCCs (88%) and a minority (18%) of VIN 3 excisions were associated with lichen sclerosus. Normal vulvar skin controls did not exhibit chromosome 17 polysomy (cells with more than four FISH signals), whereas 56% of normal vulvar skin associated with cancer did. Moreover, the frequency of polysomy significantly increased as the histological classification progressed from normal to inflammatory to neoplastic lesions. The largest mean value and variance for chromosome 17 copy number was identified in SCCs (2.4 +/- 1.0) with intermediate values identified, in decreasing order, for SCC in situ (2.1 +/- 1.0), VIN 2 (2.1 +/- 0.8), lichen sclerosus (2.0 +/- 0.5), lichen simplex chronicus (1.9 +/- 0.4), and normal skin associated with SCC (1.8 +/- 0.4) compared with control vulvar skin (1.5 +/- 0. 05). Concordance of chromosome 17 aneusomy between cancers and synchronous skin lesions was found in 48% of patients. Loss of chromosome 17 was identified 5% of all samples and was significantly associated with women with SCC in situ (HPV-related). Both DNA content and Ki-67 labeling positively and significantly correlated with mean chromosome 17 copy number (r = 0.1, P: = 0.007). A high degree of genetic instability (aneuploidy) occurs in the skin surrounding vulvar carcinomas. As these events could be detected in histologically normal skin and inflammatory lesions (lichen sclerosus), chromosomal abnormalities may be a driving force in the early stages of carcinogenesis. Differences in chromosomal patterns (loss or gain) support the concept of at least two pathways in vulvar carcinogenesis. |
| Databáze: | OpenAIRE |
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