Role of Glucocorticoids and cAMP-Mediated Repression in Limiting Corticotropin-Releasing Hormone Transcription during Stress

Autor: Greti Aguilera, Paolo Sassone-Corsi, Ying Liu, Jack D. Shepard
Přispěvatelé: Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2005
Předmět:
Male
Time Factors
Transcription
Genetic
Corticotropin-Releasing Hormone
Response element
Electrophoretic Mobility Shift Assay
RNA polymerase II
MESH: Rats
Sprague-Dawley
Rats
Sprague-Dawley
Corticotropin-releasing hormone
0302 clinical medicine
Adrenal Cortex Hormones
Transcription (biology)
MESH: Animals
In Situ Hybridization
MESH: Adrenalectomy
MESH: Chromatin Immunoprecipitation
0303 health sciences
General Neuroscience
Adrenalectomy
MESH: Restraint
Physical
MESH: Gene Expression Regulation
MESH: Corticotropin-Releasing Hormone
MESH: RNA
Heterogeneous Nuclear
hormones
hormone substitutes
and hormone antagonists
Glucocorticoid
Cellular/Molecular
medicine.drug
Restraint
Physical
Chromatin Immunoprecipitation
endocrine system
medicine.medical_specialty
MESH: Rats
Blotting
Western
Repressor
MESH: Stress
In situ hybridization
Biology
MESH: Cyclic AMP Response Element Modulator
Cyclic AMP Response Element Modulator
MESH: Adrenal Cortex Hormones
03 medical and health sciences
MESH: In Situ Hybridization
Stress
Physiological
MESH: Analysis of Variance
Internal medicine
medicine
Animals
MESH: Blotting
Western
Electrophoretic mobility shift assay
RNA
Messenger
Glucocorticoids
MESH: RNA
Messenger
030304 developmental biology
Analysis of Variance
MESH: Transcription
Genetic
MESH: Time Factors
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
MESH: Male
Rats
Endocrinology
Gene Expression Regulation
nervous system
MESH: Electrophoretic Mobility Shift Assay
biology.protein
RNA
Heterogeneous Nuclear
MESH: Glucocorticoids
MESH: Paraventricular Hypothalamic Nucleus
030217 neurology & neurosurgery
Paraventricular Hypothalamic Nucleus
Zdroj: Journal of Neuroscience
Journal of Neuroscience, Society for Neuroscience, 2005, 25 (16), pp.4073-81. ⟨10.1523/JNEUROSCI.0122-05.2005⟩
ISSN: 1529-2401
0270-6474
DOI: 10.1523/jneurosci.0122-05.2005
Popis: The role of glucocorticoids and the repressor isoform of cAMP response element (CRE) modulator (CREM), inducible cAMP early repressor (ICER), in limiting corticotropin-releasing hormone (CRH) transcription during restraint stress were examined in both intact and adrenalectomized rats receiving glucocorticoid replacement. CRH primary transcript, measured by intronicin situhybridization, increased after 30 min of restraint and returned to basal levels by 90 min, despite the persistent stressor. The decline was independent of circulating glucocorticoids, because adrenalectomized rats displayed an identical pattern. ICER mRNA in the hypothalamic paraventricular nucleus (PVN) increased after 30 min and remained elevated for up to 4 h in a glucocorticoid-independent manner. Western blot and electrophoretic mobility shift assay analyses showed increases in endogenous ICER in the PVN of rats subjected to restraint stress for 3 h. Chromatin immunoprecipitation assays showed the recruitment of CREM by the CRH CRE in conjunction with decreases in RNA polymerase II (Pol II) binding in the PVN region of rats restrained for 3 h. These data show that stress-induced glucocorticoids do not mediate the limitation of CRH transcription. Furthermore, the ability of CREM to bind the CRH CRE and the time relationship between elevated CREM and reduced Pol II recruitment by the CRH promoter suggest that inhibitory isoforms of CREM induced during stress contribute to the decline in CRH gene transcription during persistent stimulation.
Databáze: OpenAIRE
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