Antidiabetic and Renoprotective Effects of Coffea arabica Pulp Aqueous Extract through Preserving Organic Cation Transport System Mediated Oxidative Stress Pathway in Experimental Type 2 Diabetic Rats
| Autor: | Metee Jinakote, Tipthida Pasachan, Chutima Srimaroeng, Atcharaporn Ontawong, Acharaporn Duangjai, Doungporn Amornlerdpison, Manussabhorn Phatsara, Oranit Boonphang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Organic cation transport
Antioxidant antioxidant endocrine system diseases antidiabetic effect medicine.medical_treatment Pharmaceutical Science Pharmacology Analytical Chemistry Lipid peroxidation lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance lcsh:Organic chemistry In vivo Drug Discovery medicine renal organic cation transport Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences Kidney urogenital system Organic Chemistry nutritional and metabolic diseases Streptozotocin medicine.disease medicine.anatomical_structure chemistry Chemistry (miscellaneous) Polyphenol 030220 oncology & carcinogenesis diabetes mellitus Molecular Medicine medicine.drug Coffea arabica pulp |
| Zdroj: | Molecules Volume 26 Issue 7 Molecules, Vol 26, Iss 1907, p 1907 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26071907 |
| Popis: | Coffea arabica pulp (CP) is a by-product of coffee processing. CP contains polyphenols that have exhibited beneficial effects, including antioxidant and lipid-lowering effects, as well as enhanced insulin sensitivity, in in vitro and in vivo models. How polyphenols, as found in CP aqueous extract (CPE), affect type 2 diabetes (T2D) has not been investigated. Thus, the present study examined the potential antidiabetic, antioxidant, and renoprotective effects of CPE-rich polyphenols, using an experimental model of T2D in rats induced by a high-fat diet and a single low dose of streptozotocin. The T2D rats received either 1000 mg/kg body weight (BW) of CPE, 30 mg/kg BW of metformin (Met), or a combination treatment (CPE + Met) for 3 months. Plasma parameters, kidney morphology and function, and renal organic transport were determined. Significant hyperglycemia, hypertriglyceridemia, insulin resistance, increased renal lipid content and lipid peroxidation, and morphological kidney changes related to T2D were restored by both CPE and CPE + Met treatments. Additionally, the renal uptake of organic cation, 3H-1-methyl-4-phenylpyridinium (MPP+), was reduced in T2D, while transport was restored by CPE and CPE + Met, through an up-regulation of antioxidant genes and protein kinase Cα deactivation. Thus, CPE has antidiabetic and antioxidant effects that potentially ameliorate kidney function in T2D by preserving renal organic cation transport through an oxidative stress pathway. |
| Databáze: | OpenAIRE |
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