Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells
| Autor: | Béla Völgyi, Alma Ganczer, Tamás Kovács-Öller, Miklós Nyitrai, Boglárka Balogh, Ádám J Tengölics, Edina Szabó-Meleg, Gergely Szarka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Retinal Ganglion Cells retina ganglion cell calcium buffer protein Retinal ganglion Calbindin Article 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine topography calbindin mental disorders parvalbumin expression medicine Animals Cluster Analysis natural sciences lcsh:QH301-705.5 Retina biology musculoskeletal neural and ocular physiology calretinin Calcium-Binding Proteins Proteins Retinal General Medicine eye diseases Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system chemistry lcsh:Biology (General) biology.protein Immunohistochemistry Soma Calcium sense organs Calretinin 030217 neurology & neurosurgery Parvalbumin |
| Zdroj: | Cells Cells, Vol 9, Iss 4, p 792 (2020) Volume 9 Issue 4 |
| ISSN: | 2073-4409 |
| Popis: | The most prevalent Ca2+-buffer proteins (CaBPs: parvalbumin&mdash PV calbindin&mdash CaB calretinin&mdash CaR) are widely expressed by various neurons throughout the brain, including the retinal ganglion cells (RGCs). Even though their retinal expression has been extensively studied, a coherent assessment of topographical variations is missing. To examine this, we performed immunohistochemistry (IHC) in mouse retinas. We found variability in the expression levels and cell numbers for CaR, with stronger and more numerous labels in the dorso-central area. CaBP+ cells contributed to RGCs with all soma sizes, indicating heterogeneity. We separated four to nine RGC clusters in each area based on expression levels and soma sizes. Besides the overall high variety in cluster number and size, the peripheral half of the temporal retina showed the greatest cluster number, indicating a better separation of RGC subtypes there. Multiple labels showed that 39% of the RGCs showed positivity for a single CaBP, 30% expressed two CaBPs, 25% showed no CaBP expression, and 6% expressed all three proteins. Finally, we observed an inverse relation between CaB and CaR expression levels in CaB/CaR dual- and CaB/CaR/PV triple-labeled RGCs, suggesting a mutual complementary function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |