Gene frequencies of the HNA-4a and -5a neutrophil antigens in Brazilian persons and a new polymerase chain reaction-restriction fragment length polymorphism method for HNA-5a genotyping
| Autor: | José Orlando Bordin, Angela M. M. I. Norcia, José Daniel Braz Cardone, Akemi Kuroda Chiba, João P. B. Vieira-Filho |
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| Rok vydání: | 2006 |
| Předmět: |
Isoantigens
Genotype Neutrophils Immunology Population Single-nucleotide polymorphism Biology Polymerase Chain Reaction Gene Frequency Polymorphism (computer science) Immunology and Allergy Humans education Genotyping Allele frequency Alleles Genetics education.field_of_study Polymorphism Genetic Indians South American Hematology Molecular biology Genotype frequency Restriction fragment length polymorphism Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Transfusion. 46(9) |
| ISSN: | 0041-1132 |
| Popis: | BACKGROUND: The HNA-4a (Mart) and HNA-5a (Ond) antigens are polymorphic variants of αM (CD11b) and αL (CD11a) subunits of the β2-integrin, and are associated with single nucleotide polymorphisms (SNP) leading to amino acid dimorphisms. HNA-4a has been linked to alloimmune neonatal neutropenia, but the HNA-5a clinical significance is unclear. STUDY DESIGN AND METHODS: Using a polymerase chain reaction (PCR) with sequence-specific primers, the frequency of HNA-4a among 121 Brazilian blood donors and 114 Amazon Indians was determined. A PCR-restriction fragment length polymorphism (RFLP) method for HNA-5a genotyping was developed, and the gene frequencies of this antigen were determined among 123 blood donors and 114 Indians. To validate the genotyping method, the amplified DNA from six previously obtained samples (two of each genotype) was sequenced. RESULTS: The HNA-4a (+/+), HNA-4a (+/−), and HNA-4a (−/−) genotype frequencies of blood donors (0.686, 0.273, 0.041) and Indians (1.000, 0.000, 0.000) were different (p |
| Databáze: | OpenAIRE |
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