Prevalence of free living amoeba in the domestic waters reservoirs in Sfax, Tunisia
| Autor: | F. Dendana, H. Trabelsi, H. Sellami, A. Ayadi, F. Makni, S. Neji, J. Feki, S. Kammoun, F. Cheikhrouhou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Veterinary medicine Tunisia food.ingredient 030106 microbiology Immunology Water source Biocompatible Materials Fresh Water Biology Azure Stains 03 medical and health sciences Opportunistic pathogen food Methyl Green Water Supply Trichrome Agar Prospective Studies Amoeba Cellulose Coloring Agents Free living amoeba General Medicine 030104 developmental biology Infectious Diseases Eosine Yellowish-(YS) Parasitology Cellulose acetate membrane Azo Compounds Filtration |
| Zdroj: | Experimental Parasitology. 193:1-4 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2018.07.007 |
| Popis: | Free living amoebas (FLA) are opportunistic pathogen found in different water sources in the environment. The aim of this study was to investigate the prevalence of free living amoeba in different samples of domestic water reserves (DWR) in Sfax region from Tunisia. It was a prospective study dealing with 486 water samples collected from different DWR. After filtration through a cellulose acetate membrane samples were cultured on non-nutrient agar and the FLA were detected and strained with Giesma, Trichrome and red nuclear stain for morphological and morphotypic studies. FLA were found in 62% of samples. The Acanthopodial morphotype was detected in 43%, Polytactic (38%), Monotactic (28%), Fan-shaped (17%), Rugose (11%), Dactilopodial (10%) and Eruptive (9%). These results demonstrate that domestic water reserves are a significant source of the FLA and maintenance of DWR is recommended. |
| Databáze: | OpenAIRE |
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