Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids
| Autor: | Laurence Lins, Laetitia Fouillen, Corinne Buré, Patricia Gerbeau-Pissot, Sébastien Mongrand, Jean-Luc Cacas, Fabienne Furt, Magali Deleu, Emmanuelle Bayer, Claire Bossard, Franck Robert, Jean-Marie Schmitter, Julien Gronnier, Véronique Germain, Françoise Simon-Plas, Emmanuel Maes, Yoann Rombouts, Kevin Grosjean, Jeannine Lherminier, Stéphanie Cluzet |
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| Přispěvatelé: | Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de biogenèse membranaire (LBM), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biophysique Moléculaire aux Interfaces, Université de Liège, Worcester Polytechnic Institute, Université de Bordeaux (UB), Bordeaux Metabolome Facility-MetaboHUB [ANR-11-INBS-0010], ARC FIELD project Finding Interesting Elicitor Lipids, Fonds Speciaux pour la Recherche, University of Liege, Belgian Funds for Scientific Research, Tres Grande Infrastructure de Recherche-Resonance Magnetique Nucleaire-Tres Hauts Champs Centre National de la Recherche Scientifique [Fr3050], Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular 0106 biological sciences 0301 basic medicine Physiology [SDV]Life Sciences [q-bio] Membrane lipids Nicotiana tabacum Cell Culture Techniques Membrane biology macromolecular substances Plant Science Biology 01 natural sciences Glycosphingolipids Cell membrane Membrane Lipids 03 medical and health sciences chemistry.chemical_compound Membrane Microdomains Tobacco Genetics medicine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Inositol Glycosyl cardiovascular diseases Sphingolipids Microscopy Confocal Cell Membrane Fatty Acids technology industry and agriculture Phytosterols Articles Raft biology.organism_classification Sphingolipid Plant Leaves 030104 developmental biology medicine.anatomical_structure Biochemistry chemistry [SDE]Environmental Sciences cardiovascular system lipids (amino acids peptides and proteins) 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, 2016, 170 (1), pp.367-384. ⟨10.1104/pp.15.00564⟩ Plant Physiology, American Society of Plant Biologists, 2016, 170 (1), pp.367-384. ⟨10.1104/pp.15.00564⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.15.00564 |
| Popis: | International audience; The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 +/- 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed. |
| Databáze: | OpenAIRE |
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