DNA sequence of the skeletal muscle calcium release channel cDNA and verification of the Arg615----Cys615 mutation, associated with porcine malignant hyperthermia, in Norwegian landrace pigs
| Autor: | I. Harbitz, Tom Kristensen, S. Kran, M. Bosnes, W. Davies |
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| Předmět: |
Swine
Molecular Sequence Data chemistry.chemical_element Biology Calcium Arginine Polymerase Chain Reaction DNA sequencing Complementary DNA Genetics medicine Animals Cysteine Peptide sequence Base Sequence Muscles Nucleic acid sequence Malignant hyperthermia Skeletal muscle DNA General Medicine medicine.disease Molecular biology medicine.anatomical_structure chemistry Mutation Animal Science and Zoology Restriction digest Calcium Channels Malignant Hyperthermia |
| Zdroj: | Europe PubMed Central |
| Popis: | Summary. Porcine calcium release channel (CRC) cDNA from skeletal muscle has been cloned and sequenced. The deduced amino acid sequence showed 97% identity to the corresponding rabbit and human sequences. Using oligonucleotide primers based on the nucleotide sequence, CRC cDNA fragments from seven pigs representing HALNN, HALNn and HALNn genotypes have been amplified. Sequencing and restriction digestion of the amplified cDNA confirm that the reported C T mutation, which gives rise to Arg615 Cys615 change in the calcium release channel, is associated with the halothane sensitive allele in Norwegian Landrace pigs. The mutation may alter the reactivity of a neighbouring serine residue which is potentially phosphorylated. |
| Databáze: | OpenAIRE |
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