Alienimonas californiensis gen. nov. sp. nov., a novel Planctomycete isolated from the kelp forest in Monterey Bay
Autor: | Rob Mesman, Stijn H Peeters, Mike S. M. Jetten, Manfred Rohde, Christian Boedeker, Patrick Rast, Alje S Boersma, Nicolai Kallscheuer, Christian Jogler, Mareike Jogler, Sandra Wiegand, Anja Heuer |
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Rok vydání: | 2019 |
Předmět: |
DNA
Bacterial Cell division Primary metabolism Forests Microbiology 03 medical and health sciences Genus RNA Ribosomal 16S Botany Axenic Molecular Biology Phylogeny 030304 developmental biology 0303 health sciences biology Phylogenetic tree 030306 microbiology Planctomycetes Carbohydrate active enzymes Marine bacteria Fatty Acids General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Kelp forest Bacterial Typing Techniques Type species Planctomycetales Kelp Bays Ecological Microbiology Macrocystis pyrifera |
Zdroj: | Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 113, pp. 1751-1766 Antonie van Leeuwenhoek Netherlands Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 113, 1751-1766 |
ISSN: | 0003-6072 |
Popis: | Planctomycetes are environmentally and biotechnologically important bacteria and are often found in association with nutrient-rich (marine) surfaces. To allow a more comprehensive understanding of planctomycetal lifestyle and physiology we aimed at expanding the collection of axenic cultures with new isolates. Here, we describe the isolation and genomic and physiological characterisation of strain CA12T obtained from giant bladder kelp (Macrocystis pyrifera) in Monterey Bay, California, USA. 16S rRNA gene sequence and whole genome-based phylogenetic analysis showed that strain CA12T clusters within the family Planctomycetaceae and that it has a high 16S rRNA sequence similarity (82.3%) to Planctomicrobium piriforme DSM 26348T. The genome of strain CA12T has a length of 5,475,215 bp and a G+C content of 70.1%. The highest growth rates were observed at 27 °C and pH 7.5. Using different microscopic methods, we could show that CA12T is able to divide by consecutive polar budding, without completing a characteristic planctomycetal lifestyle switch. Based on our data, we suggest that the isolated strain represents a novel species within a novel genus. We thus propose the name Alienimonas gen. nov. with Alienimonas californiensis sp. nov. as type species of the novel genus and CA12T as type strain of the novel species. |
Databáze: | OpenAIRE |
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