3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: from design to evaluation
| Autor: | Maximilian Breitfeld, Detlev Rasch, David Vorländer, Hendrik Ostsieker, Gregor D. Wehinger, Lasse Jannis Frey, Janina Bahnemann, Rainer Krull, Jan-Luca Lohse, Jan-Hendrik Grosch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
System Materials science Science 3D printing Oxygen-Transfer 02 engineering and technology Growth Computational fluid dynamics 01 natural sciences Homogenization (chemistry) Column (database) Article Mass-Transfer Engineering 010608 biotechnology Microsystem ddc:570 ddc:6 Saccharomyces-Cerevisiae Veröffentlichung der TU Braunschweig ddc:62 Process engineering Dewey Decimal Classification::500 | Naturwissenschaften Flexibility (engineering) Multidisciplinary business.industry Process (computing) Modular design 021001 nanoscience & nanotechnology Dewey Decimal Classification::600 | Technik Glucose Medicine ddc:500 Publikationsfonds der TU Braunschweig 0210 nano-technology business ddc:600 Biotechnology |
| Zdroj: | Scientific Reports Scientific Reports 11 (2021), Nr. 1 Scientific reports, 11, Article number: 7276 (2021)--Sci Rep--http://www.bibliothek.uni-regensburg.de/ezeit/?2615211--https://www.nature.com/srep/--2045-2322--2045-2322 Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| Popis: | With the technological advances in 3D printing technology, which are associated with ever-increasing printing resolution, additive manufacturing is now increasingly being used for rapid manufacturing of complex devices including microsystems development for laboratory applications. Personalized experimental devices or entire bioreactors of high complexity can be manufactured within few hours from start to finish. This study presents a customized 3D-printed micro bubble column reactor (3D-µBCR), which can be used for the cultivation of microorganisms (e.g., Saccharomyces cerevisiae) and allows online-monitoring of process parameters through integrated microsensor technology. The modular 3D-µBCR achieves rapid homogenization in less than 1 s and high oxygen transfer with kLa values up to 788 h−1 and is able to monitor biomass, pH, and DOT in the fluid phase, as well as CO2 and O2 in the gas phase. By extensive comparison of different reactor designs, the influence of the geometry on the resulting hydrodynamics was investigated. In order to quantify local flow patterns in the fluid, a three-dimensional and transient multiphase Computational Fluid Dynamics model was successfully developed and applied. The presented 3D-µBCR shows enormous potential for experimental parallelization and enables a high level of flexibility in reactor design, which can support versatile process development. |
| Databáze: | OpenAIRE |
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