Head injury profoundly affects gut microbiota homeostasis: Results of a pilot study

Autor: Christine Charrueau, Anne-Judith Waligora-Dupriet, Luc Cynober, C. Choisy, Christophe Moinard, Sophie Lafleur, Marie-José Butel
Přispěvatelé: Ecosystème intestinal, probiotiques, antibiotiques (EA 4065), Université Paris Descartes - Paris 5 (UPD5), Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université Paris Descartes - Faculté de Pharmacie de Paris (UPD5 Pharmacie), Université Sorbonne Paris Cité (USPC), Laboratoire de Biologie de la Nutrition, EA4466, Faculté de Pharmacie, Service de biochimie et de génétique moléculaire [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), grant from Nestle Health Science (Noisiel, France)., Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP]
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Endocrinology
Diabetes and Metabolism
[SDV]Life Sciences [q-bio]
Colony Count
Microbial
Prevotella
Pilot Projects
Gut flora
Gastroenterology
Rats
Sprague-Dawley
Feces
0302 clinical medicine
Lactobacillus
Bacteroides
Craniocerebral Trauma
Homeostasis
Cecum
2. Zero hunger
Colony-forming unit
Nutrition and Dietetics
biology
3. Good health
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Anaerobic exercise
medicine.medical_specialty
Gut microbiota
03 medical and health sciences
Internal medicine
medicine
Escherichia coli
Animals
Clostridium
030208 emergency & critical care medicine
biology.organism_classification
medicine.disease
Head trauma
Gastrointestinal Microbiome
Rats
Disease Models
Animal
Parenteral nutrition
Immunology
Dysbiosis
Bifidobacterium
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
030217 neurology & neurosurgery
Leuconostoc
Zdroj: Nutrition
Nutrition, Elsevier, 2018, 45, pp.104-107. ⟨10.1016/j.nut.2017.06.026⟩
ISSN: 0899-9007
DOI: 10.1016/j.nut.2017.06.026⟩
Popis: International audience; Objectives: Head injury (HI) induces a hypercatabolic state, dysimmunity, and septic complications that increase morbidity and mortality. Although compromised immune function is usually incriminated in infection occurrence, gut dysbiosis could also be involved in this phenomenon and, to our knowledge, has never been considered. To assess if HI could affect microbiota, we explored the impact of HI on intestinal microbiota in a rodent model of fluid percussion.Methods: Nineteen rats were randomly assigned to two groups: Healthy rats fed ad libitum (n ¼ 7) and HI rats (n ¼ 12), which received standard enteral nutrition for 4 d. Four days after HI, rats were euthanized and cecal contents were sampled. Cecal microbiota was assessed using real-timequantitative polymerase chain reaction.Results: HI significantly decreased the cecal content of strict anaerobic groups, Bacteroides/Prevotella group (HI 8.9 versus healthy controls 9.3 median log10 colony forming units [CFU]/g, P ¼ 0.007), Clostridium cluster XIVab (HI 7.9 versus healthy controls 8.9 median log10 CFU/g, P ¼ 0.002), Lactobacillus/Leuconostoc group (HI 8.5 versus healthy controls 9.4 median log10 CFU/g, P ¼ 0.044), and Bifidobacterium sp. (HI 3.0 versus healthy controls 8.2 median log10 CFU/g, P < 0.001). In contrast, colonization by Escherichia coli was dramatically increased (HI 10.5 versus healthy controls 7.0 median log10 CFU/g, P < 0.001).Conclusions: HI profoundly modified the gut microbiota homeostasis and thus could contribute to infection in head trauma patients. These preliminary results open a new field of research in the management of patients with HI.
Databáze: OpenAIRE
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