Hirsute or Hairless? Two Proteins May Spell the Difference

Autor: Colin Jamora, Elaine Fuchs, Pedro Lee, Ryoichi Hosokawa, Pawel Kocieniewski, Mohamad Azhar, Yang Chai
Rok vydání: 2004
Předmět:
Cell signaling
Time Factors
Transcription
Genetic
QH301-705.5
MAP Kinase Signaling System
Blotting
Western
Morphogenesis
Down-Regulation
Mice
Transgenic
Cell Communication
Development
Biology
Epithelium
General Biochemistry
Genetics and Molecular Biology
Mice
Transforming Growth Factor beta2
Transforming Growth Factor beta
medicine
Animals
Biology (General)
Transcription factor
Cell Proliferation
Skin
General Immunology and Microbiology
Reverse Transcriptase Polymerase Chain Reaction
Cadherin
General Neuroscience
Gene Expression Regulation
Developmental
Cell Biology
Transforming growth factor beta
Mus (Mouse)
Cadherins
Hair follicle
Immunohistochemistry
Cell biology
Hair follicle morphogenesis
medicine.anatomical_structure
biology.protein
Snail Family Transcription Factors
Signal transduction
General Agricultural and Biological Sciences
Hair Follicle
Research Article
Signal Transduction
Transcription Factors
Zdroj: PLoS Biology
PLoS Biology, Vol 3, Iss 1, p e11 (2005)
ISSN: 1545-7885
Popis: In a common theme of organogenesis, certain cells within a multipotent epithelial sheet exchange signals with their neighbors and develop into a bud structure. Using hair bud morphogenesis as a paradigm, we employed mutant mouse models and cultured keratinocytes to dissect the contributions of multiple extracellular cues in orchestrating adhesion dynamics and proliferation to shape the cluster of cells involved. We found that transforming growth factor β2 signaling is necessary to transiently induce the transcription factor Snail and activate the Ras-mitogen-activated protein kinase (MAPK) pathway in the bud. In the epidermis, Snail misexpression leads to hyperproliferation and a reduction in intercellular adhesion. When E-cadherin is transcriptionally down-regulated, associated adhesion proteins with dual functions in signaling are released from cell-cell contacts, a process which we demonstrate leads to Ras-MAPK activation. These studies provide insights into how multipotent cells within a sheet are stimulated to undergo transcriptional changes that result in proliferation, junctional remodeling, and bud formation. This novel signaling pathway further weaves together the web of different morphogens and downstream transcriptional events that guide hair bud formation within the developing skin.
The study of hair follicle morphogenesis provides insights into how cells within a sheet can be triggered to proliferate, remodel, and form buds - a recurring theme in development
Databáze: OpenAIRE
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