Comparative transcriptome analyses revealed key genes involved in high amylopectin biosynthesis in wheat

Autor:	Prashant Kumar, Ankita Mishra, Mohammed Saba Rahim, Vinita Sharma, Akansha Madhawan, Afsana Parveen, Vikas Fandade, Himanshu Sharma, Joy Roy
Rok vydání:	2022
Předmět:	Environmental Science (miscellaneous) Agricultural and Biological Sciences (miscellaneous) Biotechnology
Zdroj:	3 Biotech. 12(11)
ISSN:	2190-572X
Popis:	High amylopectin starch is an important modified starch for food processing industries. Despite a thorough understanding of starch biosynthesis pathway, the regulatory mechanism responsible for amylopectin biosynthesis is not well explored. The present study utilized transcriptome sequencing approach to understand the molecular basis of high amylopectin content in three high amylopectin mutant wheat lines ('TAC 6', 'TAC 358', and 'TAC 846') along with parent variety 'C 306'. Differential scanning calorimetry (DSC) of high amylopectin starch identified a high thermal transition temperature and scanning electron microscopy (SEM) revealed more spherical starch granules in mutant lines compared to parent variety. A set of 4455 differentially expressed genes (DEGs) were identified at two-fold compared to the parent variety in high amylopectin wheat mutants. At ten-fold, 279 genes, including two starch branching genes (The online version contains supplementary material available at 10.1007/s13205-022-03364-3.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5998fe9ac025a325d35e9f5c600f4faa https://pubmed.ncbi.nlm.nih.gov/36276458 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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