Novel artificial stool material for external quality assurance (EQA) on a fecal immunochemical test for hemoglobin (FIT): The confirmed utility of stable hemoglobin and an internal standard material
| Autor: | Yamada Miyu, Ryota Yasui, Ikunosuke Sakurabayashi, Katsunori Watanabe, Shizuka Takehara |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Quality Control
030213 general clinical medicine Estimated Weight Clinical Biochemistry Population Clinical Chemistry Tests Biochemistry Feces 03 medical and health sciences 0302 clinical medicine Screening programs Medicine Food science education Standard material education.field_of_study business.industry Immunochemistry Biochemistry (medical) General Medicine Reference Standards Specimen collection Fecal Immunochemical Test Occult Blood 030220 oncology & carcinogenesis Hemoglobin Colorectal Neoplasms business Quality assurance |
| Zdroj: | Clinica Chimica Acta. 483:76-81 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2018.04.021 |
| Popis: | The fecal immunochemical test for hemoglobin (FIT), which detects lower gastrointestinal bleeding, is widely accepted for population-based colorectal cancer (CRC) screening programs. However, the FIT screening process has not been standardized yet, and standardizing the pre-analytical phase and establishing an external quality assurance (EQA) program compliant with ISO requirements is urgently needed. Although there have been various attempts to establish EQA materials suitable for FIT, no materials have yet been reported to have sufficient uniformity and acceptable immunochemical stability of hemoglobin (Hb). The Health Care Technology Foundation (HECTEF; Tokyo Japan) is now developing a ready-to-use artificial stool containing Hb and an internal standard, glycerol. Accordingly, we verified the adaptability and efficacy of this material for the evaluation of the specimen collection phase of FIT. This material uniformly contained both Hb and glycerol. The glycerol allowed us to estimate the weight of the collected artificial stool and to correct the Hb concentration with the estimated weight. Furthermore, the stability of both Hb and glycerol were confirmed to be sufficient for an EQA material under appropriate storage, in-use, repeated freeze-thaw, and heated conditions. These in-house performance characteristics suggest that HECTEF artificial stool is acceptable as an EQA material for FIT. |
| Databáze: | OpenAIRE |
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