Viral Inhibition of Bacterial Phagocytosis by Human Macrophages: Redundant Role of CD36

Autor: Leidy Y Bastidas-Legarda, Zoe C. Pounce, Anna S. Tocheva, Emily C. Robinson, Tom Wilkinson, Myron Christodoulides, Ben Nicholas, Joshua C. Wallington, Grace E. Cooper, Karl J. Staples, Chiamaka Chidomere, Ratko Djukanovic, Kirstin Martin
Rok vydání: 2016
Předmět:
CD36 Antigens
0301 basic medicine
Viral Diseases
Small interfering RNA
CD36
lcsh:Medicine
Gene Expression
Pathology and Laboratory Medicine
Biochemistry
White Blood Cells
Spectrum Analysis Techniques
Animal Cells
Medicine and Health Sciences
Macrophage
Small interfering RNAs
lcsh:Science
Cells
Cultured
Gene knockdown
Multidisciplinary
biology
Pneumococcus
Transfection
Flow Cytometry
Respiratory Syncytial Viruses
Bacterial Pathogens
Nucleic acids
Streptococcus pneumoniae
Infectious Diseases
Influenza A virus
Cell Processes
Spectrophotometry
Medical Microbiology
RNA
Viral
Cytophotometry
Cellular Types
Pathogens
Research Article
Immune Cells
Phagocytosis
Immunology
Down-Regulation
Research and Analysis Methods
Microbiology
Virus
03 medical and health sciences
Macrophages
Alveolar
Genetics
Humans
RNA Viruses
Scavenger receptor
Non-coding RNA
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Blood Cells
Bacteria
Macrophages
lcsh:R
Organisms
Biology and Life Sciences
Streptococcus
Interferon-beta
Cell Biology
Molecular biology
Influenza
Gene regulation
030104 developmental biology
biology.protein
RNA
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 10, p e0163889 (2016)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0163889
Popis: Macrophages are essential to maintaining lung homoeostasis and recent work has demonstrated that influenza-infected lung macrophages downregulate their expression of the scavenger receptor CD36. This receptor has also been shown to be involved in phagocytosis of Streptococcus pneumoniae, a primary agent associated with pneumonia secondary to viral infection. The aim of this study was to investigate the role of CD36 in the effects of viral infection on macrophage phagocytic function. Human monocyte-derived macrophages (MDM) were exposed to H3N2 X31 influenza virus, M37 respiratory syncytial virus (RSV) or UV-irradiated virus. No infection of MDM was seen upon exposure to UV-irradiated virus but incubation with live X31 or M37 resulted in significant levels of viral detection by flow cytometry or RT-PCR respectively. Infection resulted in significantly diminished uptake of S. pneumoniae by MDM and significantly decreased expression of CD36 at both the cell surface and mRNA level. Concurrently, there was a significant increase in IFN? gene expression in response to infection and we observed a significant decrease in bacterial phagocytosis (p = 0.031) and CD36 gene expression (p = 0.031) by MDM cultured for 24 h in 50IU/ml IFN?. Knockdown of CD36 by siRNA resulted in decreased phagocytosis, but this was mimicked by transfection reagent alone. When MDM were incubated with CD36 blocking antibodies no effect on phagocytic ability was observed. These data indicate that autologous IFN? production by virally-infected cells can inhibit bacterial phagocytosis, but that decreased CD36 expression by these cells does not play a major role in this functional deficiency.
Databáze: OpenAIRE
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