CD82 hypomethylation is essential for tuberculosis pathogenesis via regulation of RUNX1-Rab5/22

Autor: Kiseok Jang, Ye-Ram Kim, Jin-Seung Yun, Sojin Kim, Hyun-Jung Koh, Chul-Su Yang, Sun-Young Kim, Jae-Sung Kim
Rok vydání: 2018
Předmět:
0301 basic medicine
Tumor suppressor gene
medicine.medical_treatment
Clinical Biochemistry
lcsh:Medicine
Biology
Kangai-1 Protein
Biochemistry
Phagolysosome
Article
Microbiology
lcsh:Biochemistry
Mycobacterium tuberculosis
03 medical and health sciences
Immune system
Tetraspanin
Phagosome maturation
medicine
Animals
Humans
Tuberculosis
lcsh:QD415-436
DNA (Cytosine-5-)-Methyltransferases
Promoter Regions
Genetic
Lung
Molecular Biology
rab5 GTP-Binding Proteins
Phagosome
Inflammation
Mice
Knockout
Microbial Viability
Virulence
Macrophages
lcsh:R
DNA Methylation
bacterial infections and mycoses
biology.organism_classification
Up-Regulation
Mice
Inbred C57BL
030104 developmental biology
Cytokine
rab GTP-Binding Proteins
Core Binding Factor Alpha 2 Subunit
Cytokines
Molecular Medicine
Protein Binding
Zdroj: Experimental & Molecular Medicine
Experimental and Molecular Medicine, Vol 50, Iss 5, Pp 1-15 (2018)
ISSN: 2092-6413
1226-3613
Popis: The tumor suppressor gene CD82/KAI1 is a member of the tetraspanin superfamily and organizes various membrane-based processes. Mycobacterium tuberculosis (MTB) persists in host macrophages by interfering with phagolysosome biogenesis and inflammatory responses, but the role of CD82 in controlling the intracellular survival of pathogenic mycobacteria within macrophages remains poorly understood. In this study, we demonstrated that the virulent MTB strain H37Rv (MTB Rv) induced CD82 promoter hypomethylation, resulting in CD82 expression. Targeting of the runt-related transcription factor 1 (RUNX1) by CD82 is essential for phagosome arrest via interacting with Rab5/22. This arrest is required for the intracellular growth of MTB in vitro and in vivo, but not for that of MTB H37Ra (MTB Ra) in macrophages. In addition, knockdown or knockout of CD82 or RUNX1 increased antibacterial host defense via phagolysosome biogenesis, inflammatory cytokine production, and subsequent antimicrobial activity both in vitro and in vivo. Notably, the levels of CD82 and RUNX1 in granulomas were elevated in tuberculosis (TB) patients, indicating that CD82 and RUNX1 have clinical significance in human TB. Our findings identify a previously unrecognized role of CD82 hypomethylation in the regulation of phagosome maturation, enhanced intracellular survival, and the innate host immune response to MTB. Thus, the CD82–RUNX1–Rab5/22 axis may be a previously unrecognized virulence mechanism of MTB pathogenesis.
Tuberculosis: Evading host defences The tuberculosis-causing bacterium Mycobacterium tuberculosis regulates a tumor suppressor gene in order to survive and grow in host immune cells. Chul-Su Yang and colleagues at Hanyang University, South Korea, have found that the bacterium can stimulate the expression of CD82 in macrophages by removing methyl groups from its DNA sequence. CD82’s hypomethylated region interacts with and activates proteins that interfere with the cell’s ability to mount an inflammatory response and degrade bacteria in specialized intracellular vesicles called lysosomes. The increased survival rate of CD82-deficient mice following infection with tuberculosis and the elevated levels of CD82 protein found in the inflammatory lesions of patients with tuberculosis further support a previously unrecognized role for this protein in M. tuberculosis infection. Targeting CD82-mediated signaling could be a promising approach for designing new therapeutics.
Databáze: OpenAIRE
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